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Rad51L1 Antibody (1 H3/13)

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Western Blot: Rad51L1 Antibody (1 H3/13) [NB100-176] - Western blot analysis of Rad51L1 expression in 1) HeLa, 2) HepG2 and 3) Cos 7 whole cell lysates.
Western Blot: Rad51L1 Antibody (1 H3/13) [NB100-176] - Western blot analysis of Rad51L1 expression in 1) HeLa, 2) HepG2 and 3) Cos 7 whole cell lysates.

Product Details

Summary
Reactivity Hu, Pm, Mu(-)Species Glossary
Applications WB, ICC/IF
Clone
1 H3/13
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Rad51L1 Antibody (1 H3/13) Summary

Immunogen
His-tagged human Rad51L1, overexpressed in E. coli. [UniProt# O15315]
Specificity
Does not cross-react with Rad51C, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RAD51B
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Rad51L1 antibody (1 H3/13) is useful for Western blot, where a band can be seen at ~ 42 kDa. Immunoprecipitation (-) Immunocytochemistry/Immunofluorescence was reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Rad51L1 Overexpression Lysate
Publications
Read Publications using
NB100-176 in the following applications:

Reactivity Notes

Human and primate. This antibody does not work in mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Rad51L1 Antibody (1 H3/13)

  • DNA repair protein RAD51 homolog 2
  • MGC34245
  • R51H2REC2hREC2
  • RAD51 (S. cerevisiae)-like 1
  • RAD51 homolog B
  • RAD51B
  • RAD51-like 1 (S. cerevisiae)
  • RAD51-like protein 1
  • RecA-like protein
  • recombination repair protein

Background

Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologues interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. Rad51B plays a role in meiotic recombination and/or recombinational repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Rad51 Antibody (14B4) [NB100-148] - Various whole cell extracts (70 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - staining of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.
Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - BT483 xenograft. RAD51 antibody 14B4 dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Western Blot: Rad51D Antibody (5B3/6) [NB100-178] - Rad51D detected in HEK293 lysates using a 1:1,000 dilution of NB 100-178 (purified). Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts Medical School
Immunocytochemistry/Immunofluorescence: Rad51D Antibody (5B3/6) [NB100-178] - RAD51D antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
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Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Analysis of RAD51c in 1) Hela WCE 2) HepG2 WCE 3) Cos-7 WCE 4) Hek293 WCE.
Flow Cytometry: Rad51C Antibody (2H11/6) [NB100-177] - Intracellular flow cytometric staining of 1 x 10^6 HeLa cells using Rad51C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Rad51C detected in HEK293 lysate using NB 100-177. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School.
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H00007516-M01
Western Blot: XRCC2 Antibody (2H4) [H00007516-M01] - Analysis of XRCC2 expression in transfected 293T cell line by XRCC2 monoclonal antibody (M01), clone 2H4.Lane 1: XRCC2 transfected lysate(32 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: XRCC2 Antibody (2H4) [H00007516-M01] - Analysis of monoclonal antibody to XRCC2 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: XRCC2 Antibody (2H4) [H00007516-M01] - Detection limit for recombinant GST tagged XRCC2 is approximately 0.3ng/ml as a capture antibody.
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NB100-165
Western Blot: XRCC3 Antibody [NB100-165] - (A) Human XRCC3 (hXRCC3) expression was down regulated by siRNA in human transofrmed fibroblasts. (B) XRCC3 was inactivated in human colon cancer cells by gene targeting [Yoshihara et all., 2004, EMBO J., 23 (3), 670-680]. hXRCC3 was detected by WB using XRCC3 antibody (NB100-165). The star indicates non-specific cross-reactivity of the antibody. Image provided by confirmed customer review.
Western Blot: XRCC3 Antibody [NB100-165] - Detection of XRCC3 (38 kDa) from HeLa cell extract using NB100-165 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.
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Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.
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Western Blot: HID1 Antibody (OTI2F4) [NBP2-02667] - Analysis of extracts (10ug) from 10 Human tissue by using anti-C17orf28 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Immunohistochemistry-Paraffin: HID1 Antibody (2F4) [NBP2-02667] - Staining of paraffin-embedded Human prostate tissue using anti-C17orf28 mouse monoclonal antibody.
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Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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HeLa Cell Lysate
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Species: Hu
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Western Blot: ATRX Antibody [NBP1-83077] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ATRX antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunocytochemistry/Immunofluorescence: ATRX Antibody [NBP1-83077] - Staining of human cell line A549 shows localization to nucleoplasm and nuclear bodies. Antibody staining is shown in green.
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Western Blot: HMGA2 Antibody (763) [NBP2-43640] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [763] diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: HMGA2 Antibody (763) [NBP2-43640] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGA2 protein stained by HMGA2 antibody [763] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
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Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2G1D5) [NB100-1768] - Chk1 antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.
Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis of FFPE human lung using Chk1 antibody (clone 2G1D5) at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and nuclear staining of Chk1 was observed as this is a ubiquitous cell checkpoint protein. Staining was performed by Histowiz.
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Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade.
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Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5 % SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade BRCA1 diluted by 1:500
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Western Blot: Rad1 Antibody [NB100-56320] - Analysis of Rad1 in HEK293 cell lysate using anti-Rad1 antibody. Image from verified customer review.
Western Blot: Rad1 Antibody [NB100-56320] - Western blot analysis of Rad1 in A) HeLa and B) HEK293 cell lysate using this antibody at 4 ug/ml.
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Western Blot: 53BP1 Antibody [NB100-304] - Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-53BP1 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Knockout Validated: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Immunohistochemistry-Frozen: 53BP1 Antibody [NB100-304] - Irradiated cochlear spiral ganglion cells, mouse, frozen section of fixed material. Image from verified customer review.
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Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Western blot analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.
Immunocytochemistry/Immunofluorescence: CDK2 Antibody (1A6) [NBP2-37507] - Immunofluorescence analysis of HeLa cells using CDK2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: CDK2 Antibody (1A6) [NBP2-37507] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CDK2 mouse mAb with DAB staining.
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Progesterone
R/NR3C3 Antibody [Unc ...
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Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PR-A and PR-B at approximately 90 and 118 kDa, respectively (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Progesterone R/NR3C3 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Rad50 Antibody
(13B3)
NB100-147
Western Blot: Rad50 Antibody (13B3) [NB100-147] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term diluted at 1:500.
Immunocytochemistry/Immunofluorescence: Rad50 Antibody (13B3) [NB100-147] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad50 protein stained by Rad50 antibody [13B3] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10um.
Immunohistochemistry-Paraffin: Rad50 Antibody (13B3) [NB100-147] - Human lung cancer. Rad50 stained by Rad50 antibody [13B3] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
(1 Review)
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Rad51L1 Antibody
(1 H3/13)
NB100-176
Rad51L1 Antibody (1 H3/13)
Species: Hu, Pm, Mu(-)
Applications: WB, ICC/IF

Publications for Rad51L1 Antibody (NB100-176)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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Product General Protocols

View specific protocols for Rad51L1 Antibody (NB100-176): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rad51L1 Antibody (NB100-176) (0)

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Control Lysate(s)

HeLa Whole Cell Lysate
Rad51L1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Breast Cancer and RAD51L1 Antibodies
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Bioinformatics

Gene Symbol RAD51B
Entrez
Uniprot