Rad51L1 Antibody (1 H3/13)

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Western Blot: Rad51L1 Antibody (1 H3/13) [NB100-176] - Western blot analysis of Rad51L1 expression in 1) HeLa, 2) HepG2 and 3) Cos 7 whole cell lysates.

Product Details

Summary
Reactivity Hu, Pm, Mu(-)Species Glossary
Applications WB, ICC/IF
Clone
1 H3/13
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Rad51L1 Antibody (1 H3/13) Summary

Immunogen
His-tagged human Rad51L1, overexpressed in E. coli. [UniProt# O15315]
Specificity
Does not cross-react with Rad51C, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RAD51B
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Rad51L1 antibody (1 H3/13) is useful for Western blot, where a band can be seen at ~ 42 kDa. Immunoprecipitation (-) Immunocytochemistry/Immunofluorescence was reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Rad51L1 Lysate (NBL1-15120)
Publications
Read Publications using
NB100-176 in the following applications:

Reactivity Notes

Human and primate. This antibody does not work in mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Rad51L1 Antibody (1 H3/13)

  • DNA repair protein RAD51 homolog 2
  • MGC34245
  • R51H2REC2hREC2
  • RAD51 (S. cerevisiae)-like 1
  • RAD51 homolog B
  • RAD51B
  • RAD51-like 1 (S. cerevisiae)
  • RAD51-like protein 1
  • RecA-like protein
  • recombination repair protein

Background

Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologues interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. Rad51B plays a role in meiotic recombination and/or recombinational repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rad51L1 Antibody (NB100-176)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Human
(2)
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Product General Protocols

View specific protocols for Rad51L1 Antibody (NB100-176): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rad51L1 Antibody (NB100-176) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Breast Cancer and RAD51L1 Antibodies
In the United States, breast cancer is one of the most common cancers and the second leading cause of cancer related deaths in women. According to the American Cancer Society's most recent estimates for breast cancer in the United States, there are ab...  Read full blog post.

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Bioinformatics

Gene Symbol RAD51B
Entrez
Uniprot