Rad51C Antibody (2H11/6)


Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Analysis of RAD51c in 1) Hela WCE 2) HepG2 WCE 3) Cos-7 WCE 4) Hek293 WCE.
Flow Cytometry: Rad51C Antibody (2H11/6) [NB100-177] - Intracellular flow cytometric staining of 1 x 10^6 HeLa cells using Rad51C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more
Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Rad51C detected in HEK293 lysate using NB 100-177. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School.
Simple Western: Rad51C Antibody (2H11/6) [NB100-177] - Simple Western lane view shows a specific band for Rad51C in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Reactivity Hu, Mu, Pm, YeSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP
1 mg/ml

Rad51C Antibody (2H11/6) Summary

His-tagged human Rad51C, over-expressed in E. coli. [UniProt# O43502]
Does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS (pH 7.4)
0.02% Sodium Azide
1 mg/ml
Protein G purified


  • Western Blot 1:1000
  • Simple Western 1:100
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
This Rad51C (2H11/6) antibody is useful for Flow Cytometry and Western Blot. In WB, a band can be seen at ~40 kDa. Preliminary feedback has been negative for Immunofluorescence on 4% PFA-fixed human cell lines (H1299 and MCF7). Use in IHC-P was reported in the scientific literature (PMID: 23512992).In Simple Western only 10-15 uL of the recommended dilution is used per data point.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB100-177 in the following applications:

  • 4 publications
  • IHC
    1 publication
  • 1 publication
  • IP
    2 publications
  • WB
    11 publications

Reactivity Notes

Human, mouse, yeast, and primate.

Alternate Names for Rad51C Antibody (2H11/6)

  • DNA repair protein RAD51 homolog 3
  • MGC104277
  • R51H3
  • RAD51 homolog C (S. cerevisiae)
  • RAD51 homolog C
  • RAD51L2RAD51 (S. cerevisiae) homolog C
  • RAD51-like protein 2
  • yeast RAD51 homolog 3


The Rad51 DNA repair protein is a key component of the double-strand break repair pathway and is essential for mitotic and meiotic recombination and genomic stability. Five human Rad51 homologs have been identified: XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex, or with multiple smaller complexes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rad51C Antibody (NB100-177)(21)

We have publications tested in 3 confirmed species: Human, Mouse, Yeast.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.

Filter By Application
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB100-177 Applications Species
Park JY, Singh TR, Nassar N et al. Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair. Oncogene. 2013 Oct 21 [PMID:24141787] (WB, Human) WB Human
Huang JW, Wang Y, Dhillon KK et al. Systematic Screen Identifies miRNAs That Target RAD51 and RAD51D to Enhance Chemosensitivity. Mol Cancer Res 2013 Dec [PMID:24088786] (WB, Human) WB Human
Min A, Im SA, Yoon YK et al. RAD51C-Deficient Cancer Cells Are Highly Sensitive to the PARP Inhibitor Olaparib. Mol Cancer Ther 2013 Jun [PMID:23512992] (WB, IHC-P, ICC/IF, Human) WB, IHC-P, ICC/IF Human
Jensen RB, Ozes A, Kim T et al. BRCA2 is epistatic to the RAD51 paralogs in response to DNA damage. DNA Repair (Amst) 2013 Feb 2 [PMID:23384538] (WB, Human) WB Human
Chun J, Buechelmaier ES, Powell SN. Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway. Mol Cell Biol 2013 Jan [PMID:23149936] (Human) Human
Wang Y, Huang JW, Calses P et al. MiR-96 Downregulates REV1 and RAD51 to Promote Cellular Sensitivity to Cisplatin and PARP Inhibition Cancer Res 2012 Aug 15 [PMID:22761336] (WB, Human) WB Human
Mao Z, Tian X, Van Meter M et al. Sirtuin 6 (SIRT6) rescues the decline of homologous recombination repair during replicative senescence Proc Natl Acad Sci U S A 2012 Jul 2 [PMID:22753495] (WB, Human) WB Human
Kim TH, Payne U, Zhang X, Iwanaga Y, Davey MP, Rosenbaum JT, Inman RD. Altered host:pathogen interactions conferred by the Blau syndrome mutation of NOD2. Rheumatol Int;27(3):257-62. 2007 Jan. [PMID:20413593]
Gildemeister OS, Sage JM, Knight KL. Cellular redistribution of Rad51 in response to DNA damage: novel role for Rad51C. J Biol Chem;284(46):31945-52. 2009 Nov 13. [PMID:19783859]
Shammas MA, Shmookler Reis RJ, Koley H et al. Dysfunctional homologous recombination mediates genomic instability and progression in myeloma. Blood;113(10):2290-7. 2009 Mar 5. [PMID:19050310] (ICC/IF, Human) ICC/IF Human
Show All 21 Publications.

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Product General Protocols

View specific protocols for Rad51C Antibody (NB100-177): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rad51C Antibody (NB100-177) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-177AF405
Alexa Fluor (R) 488 Labeled NB100-177AF488
Alexa Fluor (R) 647 Labeled NB100-177AF647
Alexa Fluor (R) 700 Labeled NB100-177AF700
Allophycocyanin Labeled NB100-177APC
Biotin Labeled NB100-177B
DyLight 350 Labeled NB100-177UV
DyLight 405 Labeled NB100-177V
DyLight 405LS Labeled NB100-177V3
DyLight 488 Labeled NB100-177G
DyLight 550 Labeled NB100-177R
DyLight 650 Labeled NB100-177C
DyLight 680 Labeled NB100-177FR
DyLight 755 Labeled NB100-177IR
FITC Labeled NB100-177F
HRP Labeled NB100-177H
PE Labeled NB100-177PE
PerCP Labeled NB100-177PCP

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Contact Information

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Gene Symbol RAD51C

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