Rad51C Antibody (2H11/6) - BSA Free

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Biological Strategies: Western Blot: Rad51C Antibody (2H11/6) - BSA Free [NB100-177] - The expression of DNA damage-responsive proteins was measured by western blot analysis following treatment with olaparib and ...read more
Flow Cytometry: Rad51C Antibody (2H11/6) [NB100-177] - Intracellular flow cytometric staining of 1 x 10^6 HeLa cells using Rad51C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more
Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Rad51C detected in HEK293 lysate using NB 100-177. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School.
Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Analysis of RAD51c in 1) Hela WCE 2) HepG2 WCE 3) Cos-7 WCE 4) Hek293 WCE.
Simple Western: Rad51C Antibody (2H11/6) [NB100-177] - Lane view shows a specific band for Rad51C in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Histone deacetylase (HDAC) inhibition enhances olaparib-induced DNA damage accumulation. (B) The expression of DNA damage-responsive proteins was measured by western blot analysis following treatment with olaparib and ...read more

Product Details

Summary
Reactivity Hu, Mu, Pm, YeSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
2H11/6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Rad51C Antibody (2H11/6) - BSA Free Summary

Immunogen
His-tagged human Rad51C, over-expressed in E. coli. [UniProt# O43502]
Localization
Nuclear
Specificity
Does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RAD51C
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 23512992)
  • Immunoprecipitation
  • Simple Western 1:100
  • Western Blot 1:1000
Application Notes
In WB, a band can be seen at approx. 40 kDa. Preliminary feedback has been negative for Immunofluorescence on 4% PFA-fixed human cell lines (H1299 and MCF7).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Publications
Read Publications using
NB100-177 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Rad51C Antibody (2H11/6) - BSA Free

  • BROVCA3
  • DNA repair protein RAD51 homolog 3
  • FANCO
  • MGC104277
  • R51H3
  • RAD51 homolog C (S. cerevisiae)
  • RAD51 homolog C
  • RAD51L2RAD51 (S. cerevisiae) homolog C
  • RAD51-like protein 2
  • yeast RAD51 homolog 3

Background

The Rad51 DNA repair protein is a key component of the double-strand break repair pathway and is essential for mitotic and meiotic recombination and genomic stability. Five human Rad51 homologs have been identified: XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex, or with multiple smaller complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rad51C Antibody (NB100-177)(35)

We have publications tested in 3 confirmed species: Human, Mouse, Yeast.

We have publications tested in 6 applications: B/N, ICC/IF, IF/IHC, IHC-P, IP, WB.


Filter By Application
B/N
(1)
ICC/IF
(5)
IF/IHC
(1)
IHC-P
(1)
IP
(4)
WB
(20)
All Applications
Filter By Species
Human
(19)
Mouse
(3)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using NB100-177 Applications Species
Longo MA, Roy S, Chen Y et al. RAD51C-XRCC3 structure and cancer patient mutations define DNA replication roles Nature communications 2023-07-24 [PMID: 37488098] (IP) IP
Jamal K, Galbiati A, Armenia J et al. Drug-gene Interaction Screens Coupled to Tumor Data Analyses Identify the Most Clinically Relevant Cancer Vulnerabilities Driving Sensitivity to PARP Inhibition Cancer Research Communications 2022-10-21 [PMID: 36969741] (B/N, WB) B/N, WB
Tomaszowski KH, Roy S, Guerrero C et al. Hypomorphic Brca2 and Rad51c double mutant mice display Fanconi anemia, cancer and polygenic replication stress Nature communications 2023-03-11 [PMID: 36906610] (WB, Mouse) WB Mouse
Matsuzaki K, Kumatoriya K, Tando M et al. Polyphenols from persimmon fruit attenuate acetaldehyde-induced DNA double-strand breaks by scavenging acetaldehyde Scientific reports 2022-06-18 [PMID: 35717470]
Kusi M, Zand M, Lin LL et al. 2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity Cell reports 2022-01-11 [PMID: 35021081] (WB) WB
Kanakkanthara A, Hou X, Ekstrom TL et al. Repurposing Ceritinib Induces DNA Damage and Enhances PARP Inhibitor Responses in High-Grade Serous Ovarian Carcinoma Cancer Research 2022-01-15 [PMID: 34810199] (WB) WB
Hurley Rm, Mcgehee Cd, Nesic K Et Al. Characterization of a RAD51C-silenced high-grade serous ovarian cancer model during development of PARP inhibitor resistance NAR cancer 2021-09-01 [PMID: 34316715]
Mohan M, Akula D, Dhillon A et al. Human RAD51 paralogue RAD51C fosters repair of alkylated DNA by interacting with the ALKBH3 demethylase Nucleic Acids Res. 2019-10-23 [PMID: 31642493] (IP, Human) IP Human
Parmar K, Kochupurakkal BS, Lazaro JB et al. The CHK1 Inhibitor Prexasertib Exhibits Monotherapy Activity in High-Grade Serous Ovarian Cancer Models and Sensitizes to PARP Inhibition Clin. Cancer Res. 2019-08-13 [PMID: 31409614] (WB, Human) WB Human
Wu D, Huang CJ, Jiao XF et al. Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells Oncotarget. 2017-10-24 [PMID: 29179463] (WB, Mouse) WB Mouse
Show All 35 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAD51C