Rad51C Antibody (2H11/6)

Images

 
Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Analysis of RAD51c in 1) Hela WCE 2) HepG2 WCE 3) Cos-7 WCE 4) Hek293 WCE.
Flow Cytometry: Rad51C Antibody (2H11/6) [NB100-177] - Intracellular flow cytometric staining of 1 x 10^6 HeLa cells using Rad51C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more
Western Blot: Rad51C Antibody (2H11/6) [NB100-177] - Rad51C detected in HEK293 lysate using NB 100-177. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School.
Simple Western: Rad51C Antibody (2H11/6) [NB100-177] - Lane view shows a specific band for Rad51C in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Pm, YeSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
2H11/6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Rad51C Antibody (2H11/6) Summary

Immunogen
His-tagged human Rad51C, over-expressed in E. coli. [UniProt# O43502]
Localization
Nuclear
Specificity
Does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RAD51C
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • CyTOF-ready
Application Notes
This Rad51C (2H11/6) antibody is useful for Flow Cytometry and Western Blot. In WB, a band can be seen at approx. 40 kDa. Preliminary feedback has been negative for Immunofluorescence on 4% PFA-fixed human cell lines (H1299 and MCF7). Use in IHC-P was reported in the scientific literature (PMID: 23512992).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Publications
Read Publications using
NB100-177 in the following applications:

  • 5 publications
  • IHC
    1 publication
  • 1 publication
  • IP
    3 publications
  • WB
    16 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Rad51C Antibody (2H11/6)

  • BROVCA3
  • DNA repair protein RAD51 homolog 3
  • FANCO
  • MGC104277
  • R51H3
  • RAD51 homolog C (S. cerevisiae)
  • RAD51 homolog C
  • RAD51L2RAD51 (S. cerevisiae) homolog C
  • RAD51-like protein 2
  • yeast RAD51 homolog 3

Background

The Rad51 DNA repair protein is a key component of the double-strand break repair pathway and is essential for mitotic and meiotic recombination and genomic stability. Five human Rad51 homologs have been identified: XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex, or with multiple smaller complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rad51C Antibody (NB100-177)(28)

We have publications tested in 3 confirmed species: Human, Mouse, Yeast.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(5)
IHC
(1)
IHC-P
(1)
IP
(3)
WB
(16)
All Applications
Filter By Species
Human
(19)
Mouse
(2)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NB100-177 Applications Species
Mohan M, Akula D, Dhillon A et al. Human RAD51 paralogue RAD51C fosters repair of alkylated DNA by interacting with the ALKBH3 demethylase Nucleic Acids Res. Oct 23 2019 [PMID: 31642493] (IP, Human) IP Human
Parmar K, Kochupurakkal BS, Lazaro JB et al. The CHK1 Inhibitor Prexasertib Exhibits Monotherapy Activity in High-Grade Serous Ovarian Cancer Models and Sensitizes to PARP Inhibition Clin. Cancer Res. Aug 13 2019 [PMID: 31409614] (WB, Human) WB Human
Wu D, Huang CJ, Jiao XF et al. Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells Oncotarget. 2017 Oct 24 [PMID: 29179463] (WB, Mouse) WB Mouse
Han J, Ruan C, Huen MSY et al. BRCA2 antagonizes classical and alternative nonhomologous end-joining to prevent gross genomic instability Nat Commun. 2017 Nov 13 [PMID: 29133916] (ICC/IF, Human) ICC/IF Human
Gayarre J, Martin-Gimeno P, Osorio A et al. Characterisation of the novel deleterious RAD51C p.Arg312Trp variant and prioritisation criteria for functional analysis of RAD51C missense changes. Br. J. Cancer Sep 26 2017 [PMID: 28829762] (WB, Human) WB Human
Lai X, Broderick R, Bergoglio V et al. MUS81 nuclease activity is essential for replication stress tolerance and chromosome segregation in BRCA2-deficient cells Nat Commun 2017 Jul 17 [PMID: 28714477] (WB, Human) WB Human
Min A, Im S A et al. Histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), enhances anti-tumor effects of the poly (ADP-ribose) polymerase (PARP) inhibitor olaparib in triple-negative breast cancer cells. Breast Cancer Res Jul 3 2015 [PMID: 25888415] (WB, Human) WB Human
Park JY, Singh TR, Nassar N et al. Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair. Oncogene. 2013 Oct 21 [PMID: 24141787] (WB, Human) WB Human
Huang JW, Wang Y, Dhillon KK et al. Systematic Screen Identifies miRNAs That Target RAD51 and RAD51D to Enhance Chemosensitivity. Mol Cancer Res 2013 Dec [PMID: 24088786] (WB, Human) WB Human
Min A, Im SA, Yoon YK et al. RAD51C-Deficient Cancer Cells Are Highly Sensitive to the PARP Inhibitor Olaparib. Mol Cancer Ther 2013 Jun [PMID: 23512992] (WB, IHC-P, ICC/IF, Human) WB, IHC-P, ICC/IF Human
Show All 28 Publications.

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Product General Protocols

View specific protocols for Rad51C Antibody (NB100-177): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rad51C Antibody (NB100-177) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-177AF350
Alexa Fluor 405 NB100-177AF405
Alexa Fluor 488 NB100-177AF488
Alexa Fluor 532 NB100-177AF532
Alexa Fluor 594 NB100-177AF594
Alexa Fluor 647 NB100-177AF647
Alexa Fluor 700 NB100-177AF700
Alexa Fluor 750 NB100-177AF750
Allophycocyanin NB100-177APC
Biotin NB100-177B
DyLight 350 NB100-177UV
DyLight 405 NB100-177V
DyLight 488 NB100-177G
DyLight 550 NB100-177R
DyLight 594 NB100-177DL594
DyLight 650 NB100-177C
DyLight 680 NB100-177FR
DyLight 755 NB100-177IR
FITC NB100-177F
HRP NB100-177H
Janelia Fluor 549 NB100-177JF549
Janelia Fluor 646 NB100-177JF646
PE NB100-177PE

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Bioinformatics

Gene Symbol RAD51C