Xanthine Oxidase Antibody (8A9) - BSA Free

Immunohistochemistry: Xanthine Oxidase Antibody (8A9) [NBP3-26250] - Image of Xanthine Oxidase Antibody (8A9) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: Xanthine Oxidase Antibody (8A9) [NBP3-26250] - Positive Western Blot detected in: Hela whole cell lysate. All lanes: Xanthine Oxidase Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 147 kDa Observed band size: 147 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 8A9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Xanthine Oxidase Antibody (8A9) - BSA Free Summary

• Description: Novus Biologicals Rabbit Xanthine Oxidase Antibody (8A9) - BSA Free (NBP3-26250) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human Xanthine Oxidase [UniProt P47989]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: XDH
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Xanthine Oxidase Antibody (8A9) - BSA Free

• EC 1.17.1.4
• EC 1.17.3.2
• EC 1.7.2.2
• xanthene dehydrogenase
• xanthine dehydrogenase
• xanthine dehydrogenase/oxidase
• Xanthine Oxidase
• xanthine oxidoreductase
• XD
• XDH
• XDHA
• XO
• XOR
• XOR)

Background

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XDH