WT1 Antibody (WT1/3477R)

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221] - FFPE human kidney. PBS instead of primary antibody. Secondary antibody only negative control. Hematoxylin counterstain.

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221] - FFPE human kidney sections. Wilm's Tumor 1 labeled using WT1 (WT1/3477R) (1?g/ml). HIER: Tris/EDTA (pH9.0). HRP-Polymer. Hematoxylin counterstain.

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/3477R) [NBP3-07221] - FFPE human kidney. Secondary antibody only negative control. Hematoxylin counterstain.

Western blot analysis of THP-1 cell lysate using WT1 antibody (WT1/3477R).

Western blot analysis of K562 cell lysate using WT1 antibody (WT1/3477R).

IHC analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma. Strong nuclear staining using WT1 antibody (WT1/3477R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: WT1/3477R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

WT1 Antibody (WT1/3477R) Summary

• Description: 200ug/ml of antibody purified by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08966)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa1-100) of human WT1 protein (exact sequence is proprietary) (Uniprot: P19544 )
• Localization: Nucleus and cytoplasm. Shuttles between nucleus and cytoplasm.
• Marker: Wilm's Tumor & Mesothelial Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: WT1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for WT1 Antibody (WT1/3477R)

• AWT1
• GUD
• NPHS4GUD
• WAGR
• Wilms tumor 1
• Wilms tumor protein
• WIT-2
• WT1
• WT33

Background

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WT1
• Uniprot:
  • P19544(Human)