EWSR1 Antibody (5H7)

Images

 
Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Analysis of different cell line lysates using EWSR1 antibody, dilution 1:1000 (Green): [1] protein standard (Red), [2] HeLa, [3] HEK293, [4] mouse NIH-3T3, [5] rat PC12, ...read more
Immunocytochemistry/ Immunofluorescence: EWSR1 Antibody (5H7) [NBP1-92686] - HeLa cell cultures stained with antibody NBP1-92686 (green) and chicken antibody to vimentin (NB300-223, red). Blue is a DNA stain, and it is ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, EqSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
5H7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

EWSR1 Antibody (5H7) Summary

Immunogen
Full length recombinant human EWSR1 expressed in and purified from E. coli. [UniProt# Q01844]
Localization
Nucleus. Cytoplasm. Cell membrane. Note: Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EWSR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
Application Notes
This EWSR1 (5H7) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western blot, where a band can be seen at approximately 80 kDa.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol (1:1)
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for EWSR1 Antibody (5H7)

  • bK984G1.4
  • Ewing sarcoma breakpoint region 1 protein
  • Ewing sarcoma breakpoint region 1
  • Ewings sarcoma EWS-Fli1 (type 1) oncogene
  • EWS oncogene
  • EWSRNA-binding protein EWS

Background

EWSR1 (Ewing sarcoma breakpoint region 1) belongs to TET family (Tls, EWSR1, TafII) of RNA-binding proteins and it is implicated in transcriptional regulation for specific genes, mRNA splicing and transcription initiation. EWSR1 has the potential to associate with basal transcription factor TFIID and RNA polymerase II complex, and is required for proper localization of aurora B during mitosis as well as for maintaining mitotic spindle integrity. EWSR1 also associates with EP300/CREBBP as coactivator and EWSR1-EP300/CREBBP facilitates FOS activation as well as HNF4 genes activation. EWSR1 activates other transcription factors such as POU4F1 (or BRN3A, 13q13) and POU5F1 (or OCT4, 6p21) which regulate differentiation of neuronal cells. EWSR1 and CCNL1 are also interacting partners of TFIP11 which is functionally related to spliceosome and involved in pre-mRNA splicing. EWSR1 is required for cell survival in CNS and performs important functions in regulating genomic integrity and in RNA/microRNA's maturation processes. EWSR1 is ubiquity expressed with dominant localization in the nucleus and rarely in cytoplasm or various subcellular compartments, depending on the methylation state of its RNA-binding domain. Several gene families, mainly encoding for transcriptional regulators, can translocate with EWSR1 and the chimeric proteins have an aberrant activity interfering with different molecular pathways crucial for cell growth, differentiation and proliferation. EWSR1 is involved in several sarcoma translocations and soft tissue myoepithelial tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EWSR1 Antibody (NBP1-92686) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-92686AF350
Alexa Fluor 405 NBP1-92686AF405
Alexa Fluor 488 NBP1-92686AF488
Alexa Fluor 532 NBP1-92686AF532
Alexa Fluor 594 NBP1-92686AF594
Alexa Fluor 647 NBP1-92686AF647
Alexa Fluor 700 NBP1-92686AF700
Alexa Fluor 750 NBP1-92686AF750
DyLight 350 NBP1-92686UV
DyLight 405 NBP1-92686V
DyLight 488 NBP1-92686G
DyLight 550 NBP1-92686R
DyLight 594 NBP1-92686DL594
DyLight 650 NBP1-92686C
DyLight 680 NBP1-92686FR
DyLight 755 NBP1-92686IR
FITC NBP1-92686F
Janelia Fluor 549 NBP1-92686JF549
Janelia Fluor 646 NBP1-92686JF646

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Bioinformatics

Gene Symbol EWSR1
Entrez
Uniprot