SRY Antibody (1G4)

Western Blot: SRY Antibody (1G4) [NBP2-37463] - Analysis using SRY mouse mAb against NTERA-2 (1) cell lysate.

Immunohistochemistry-Paraffin: SRY Antibody (1G4) [NBP2-37463] - Analysis of ovarian cancer tissues using SRY mouse mAb with DAB staining.

Flow Cytometry: SRY Antibody (1G4) [NBP2-37463] - Analysis of HepG2 cells using SRY mouse mAb (green) and negative control (red).

Western Blot: SRY Antibody (1G4) [NBP2-37463] - Analysis using SRY mAb against human SRY recombinant protein. (Expected MW is 49.4 kDa)

Immunohistochemistry-Paraffin: SRY Antibody (1G4) [NBP2-37463] - Analysis of cervical cancer tissues using SRY mouse mAb with DAB staining.

ELISA: SRY Antibody (1G4) [NBP2-37463] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
• Clone: 1G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SRY Antibody (1G4) Summary

• Immunogen: Purified recombinant fragment of human SRY (AA:1-204) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SRY
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • CyTOF-ready
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 23.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for SRY Antibody (1G4)

• essential protein for sex determination in human males
• sex determining region Y
• sex-determining region on Y
• sex-determining region Y protein
• TDFsex determining region protein
• TDY
• Testis-determining factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRY