WT1 Antibody (6F-H2) [DyLight 488]

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: 6F-H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 488

WT1 Antibody (6F-H2) [DyLight 488] Summary

• Immunogen: Human recombinant Wilms Tumor 1 protein (WT1) (residues 1-181). [Swiss-Prot: P19544]
• Localization: Nuclear, Cytoplasm
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: WT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for WT1 Antibody (6F-H2) [DyLight 488]

• AWT1
• GUD
• NPHS4GUD
• WAGR
• Wilms tumor 1
• Wilms tumor protein
• WIT-2
• WT1
• WT33

Background

Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms' tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino terminus. The Wilms' tumor protein binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WT1