WT1 Antibody (6F-H2) [DyLight 405]


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Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Please see the vial label for concentration. If unlisted please contact technical services.

WT1 Antibody (6F-H2) [DyLight 405] Summary

Human recombinant Wilms Tumor 1 protein (residues 1-181). [Swiss-Prot# P19544]
Nuclear, Cytoplasm
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
DyLight 405
DyLight 405 (A=400, E=420)
50mM Sodium Borate
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Protein G purified


Application Notes
This Wilms Tumor 1 antibody is useful for Immunohistochemistry paraffin embedded / frozen sections, Western blot and Immunoprecipitation. In WB, a doublet is observed ~55 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In immunostaining, this target may be found localized to Nucleus/nucleolus and Cytoplasm of cells (WT1 shuttles between nucleus and cytoplasm - PMID 14681305).

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa

Reactivity Notes

Human and Mouse.

Alternate Names for WT1 Antibody (6F-H2) [DyLight 405]

  • AWT1
  • GUD
  • WAGR
  • Wilms tumor 1
  • Wilms tumor protein
  • WIT-2
  • WT33


Wilms' tumor 1 (WT1) gene inactivation is responsible for almost 20% of Wilms' tumour/developmental renal tumour or nephroblastoma cases and besides playing important role in cellular development and survival, this homodimeric transcription factor has been shown to involve in certain pathologies also. WT1 belongs to EGR C2H2-type zinc-finger protein family and interacts with several proteins including WTIP, HNRNPU, U2AF2, CITED2, ZNF224, WTAP, SRY, AMER1, RBM4 and actively translating polysomes (detected in nuclear ribonucleoprotein particles) for exerting its activities. WT1 binds to DNA sequence 5'-CGCCCCCGC-3' and regulates the expression of several target genes including EPO. It plays essential role in urogenital system development and in carcinogenesis, it has been suggested as tumor suppressor as well as oncogenic protein. Defects in WT1 have been linked to Wilms tumor 1 (WT1), Frasier syndrome (FS), Denys-Drash syndrome (DDS), nephrotic syndrome type 4 (NPHS4), Meacham syndrome (MEACHS), mesothelioma malignant (MESOM).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NB110-60011B
DyLight 488 Labeled NB110-60011G
DyLight 550 Labeled NB110-60011R
DyLight 650 Labeled NB110-60011C
FITC Labeled NB110-60011F
HRP Labeled NB110-60011H

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Routine WT1 Antibody Screen Uncovers an Exciting New Cancer-Cleaning Enzyme
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Contact Information

Product PDFs


Gene Symbol WT1

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