WNK4 Antibody - BSA Free

Images

 
Western Blot: WNK4 Antibody [NB600-284] - Western Blot Image of anti-WNK4. Whole cell protein from Hek293 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP, MS, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

WNK4 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to a region of the human WNK4 protein between residues 200-300. [UniProt# Q96J92]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WNK4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Electron Microscopy reported in scientific literature (PMID 24434184)
  • Immunohistochemistry
  • Immunohistochemistry-Frozen reported in scientific literature
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 24434184)
  • Immunoprecipitation reported in scientific literature (PMID 24434184)
  • Western Blot 2 ug/ml
Application Notes
In Western blot, a band is seen at ~135-155 kDa. It is not useful for Immunocytochemistry/Immunofluorescence.
Control
Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
WNK4 Peptide (NB600-284PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB600-284 in the following applications:

Publications
Read Publications using
NB600-284 in the following applications:

  • EM
    1 publication
  • 2 publications
  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    10 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34448462).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WNK4 Antibody - BSA Free

  • EC 2.7.11
  • EC 2.7.11.1
  • PHA2B
  • PRKWNK4protein kinase, lysine deficient 4
  • Protein kinase lysine-deficient 4
  • Protein kinase with no lysine 4
  • serine/threonine-protein kinase WNK4
  • WNK lysine deficient protein kinase 4

Background

WNK4 (protein kinase with no lysine 4) is a novel serine/threonine kinase which plays critical role in regulation of electrolyte homeostasis, signal transduction, survival and proliferation. WNK4 is highly expressed in kidney, colon and skin where it forms a part of tight junction complex and interacts with/activate several proteins including SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5, NEDD4L and TRPV6. It control SLC12A3 (thiazide-sensitive Na-Cl cotransporter) activity by phosphorylation process that prevents membrane trafficking of SLC12A3. WNK4 inhibits KCNJ1 (renal K+ channel) via a kinase-independent action leading to induction of protein clearance from the cell surface by clathrin-dependent endocytosis. WNK4 acts as a molecular switch that controls NaCl reabsorption and K+ secretion for maintaining integrated homeostasis. Autophosphorylation of Ser-335 is essential for WNK4's activity whereas phosphorylation of Ser-331 also increase its activity. Mutations of WNK4 can cause pseudohypoaldosteronism type alpha (PHA alpha), an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WNK4 Antibody (NB600-284)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: EM, IF/IHC, IHC-Fr, IHC-P, IP, WB.


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EM
(1)
IF/IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
IP
(1)
WB
(10)
All Applications
Filter By Species
Human
(4)
Mouse
(7)
Rat
(5)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB600-284 Applications Species
Yang HJ, Kim MJ, Kim SS, Cho YW Melatonin modulates nitric oxide-regulated WNK-SPAK/OSR1-NKCC1 signaling in dorsal raphe nucleus of rats The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2021-09-01 [PMID: 34448462] (WB, Rat) WB Rat
Zhang Y, Ye L, Yuan H, Duan D CFTR plays an important role in the regulation of vascular resistance and high-fructose/salt-diet induced hypertension in mice Journal of Cystic Fibrosis 2020-12-01 [PMID: 33279469] (WB, Mouse) WB Mouse
Frame AA, Puleo F, Kim K et al. Sympathetic regulation of the NCC in norepinephrine-evoked salt-sensitive hypertension in Sprague-Dawley rats Am. J. Physiol. Renal Physiol. 2019-10-14 [PMID: 31608673] (Rat) Rat
Kim MJ, Yang HJ, Kim Y et al. Role of nitric oxide and WNK-SPAK/OSR1-KCC2 signaling in daily changes in GABAergic inhibition in the rat dorsal raphe neurons. Neuropharmacology. 2018-03-27 [PMID: 29596900] (Rat) Rat
Huguet L, Le Dudal M, Livrozet M et al. High frequency and wide range of human kidney papillary crystalline plugs. Urolithiasis 2017-12-12 [PMID: 29234857] (IF/IHC, Human) IF/IHC Human
Li J, Hatano R, Xu S et al. Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl-cotransporter activity and expression in male and female mice. Am. J. Physiol. Renal Physiol. 2017-08-01 [PMID: 28566500] (WB, Mouse) WB Mouse
Bhuiyan M, Song S, Yuan H et al. WNK-Cab39-NKCC1 signaling increases the susceptibility to ischemic brain damage in hypertensive rats. J Cereb Blood Flow Metab 2017-01-01 [PMID: 27798271] (WB, Rat) WB Rat
Roy A, Goodman J H et al. Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing. Am J Physiol Renal Physiol 2015-02-15 [PMID: 25477473] (WB, Human) WB Human
Andrukhova O, Smorodchenko A, Egerbacher M et al. FGF23 promotes renal calcium reabsorption through the TRPV5 channel. EMBO J. 2014-02-03 [PMID: 24434184] (IP, IHC-P, EM, WB, Mouse) IP, IHC-P, EM, WB Mouse
Ohno M, Uchida K, Ohashi T et al. Immunolocalization of WNK4 in mouse kidney. Histochem Cell Biol. 2011-06-10 [PMID: 21660484] (WB, Mouse) WB Mouse
Show All 14 Publications.

Review for WNK4 Antibody (NB600-284) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB600-284:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Rat 12/13/2017
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Summary

ApplicationWestern Blot
Sample TestedBrain (cerebral cortex)
SpeciesRat
LotB-2

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Verified Customer
12/13/2017
Application: WB
Species: Rat

Bioinformatics

Gene Symbol WNK4