WNK4 Antibody

Western Blot: WNK4 Antibody [NB600-284] - Western Blot Image of anti-WNK4. Whole cell protein from Hek293 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WNK4 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence

• Reactivity: Hu, Mu, Rt
• Applications: WB, EM, IHC, IHC-Fr, IHC-P, IP, ICC/IF (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

WNK4 Antibody Summary

• Immunogen: A synthetic peptide made to a region of the human WNK4 protein between residues 200-300. [UniProt# Q96J92]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WNK4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
  • Electron Microscopy
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
• Application Notes: This WNK4 antibody is useful for Western blot where a band is seen at ~135-155 kDa. Immunohistochemistry-Frozen was reported in scientific literature. It is not useful for Immunocytochemistry/Immunofluorescence. Use in Electron Microscopy reported in scientific literature (PMID 24434184) Use in Immunohistochemistry-paraffin reported in scientific literature (PMID 24434184). Use in immunoprecipitation reported in scientific literature (PMID: 24434184).
• Control:

  Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  WNK4 Peptide (NB600-284PEP)

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WNK4 Antibody

• EC 2.7.11
• EC 2.7.11.1
• PHA2B
• PRKWNK4protein kinase, lysine deficient 4
• Protein kinase lysine-deficient 4
• Protein kinase with no lysine 4
• serine/threonine-protein kinase WNK4
• WNK lysine deficient protein kinase 4

Background

WNK4 (protein kinase with no lysine 4) is a novel serine/threonine kinase which plays critical role in regulation of electrolyte homeostasis, signal transduction, survival and proliferation. WNK4 is highly expressed in kidney, colon and skin where it forms a part of tight junction complex and interacts with/activate several proteins including SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5, NEDD4L and TRPV6. It control SLC12A3 (thiazide-sensitive Na-Cl cotransporter) activity by phosphorylation process that prevents membrane trafficking of SLC12A3. WNK4 inhibits KCNJ1 (renal K+ channel) via a kinase-independent action leading to induction of protein clearance from the cell surface by clathrin-dependent endocytosis. WNK4 acts as a molecular switch that controls NaCl reabsorption and K+ secretion for maintaining integrated homeostasis. Autophosphorylation of Ser-335 is essential for WNK4's activity whereas phosphorylation of Ser-331 also increase its activity. Mutations of WNK4 can cause pseudohypoaldosteronism type alpha (PHA alpha), an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WNK4 Antibody (NB600-284)(13)

Publications using NB600-284

• Zhang Y, Ye L, Yuan H, Duan D CFTR plays an important role in the regulation of vascular resistance and high-fructose/salt-diet induced hypertension in mice Journal of Cystic Fibrosis Dec 1 2020 [PMID: 33279469] (WB, Mouse)
• Frame AA, Puleo F, Kim K et al. Sympathetic regulation of the NCC in norepinephrine-evoked salt-sensitive hypertension in Sprague-Dawley rats Am. J. Physiol. Renal Physiol. 2019 Oct 14 [PMID: 31608673] (Rat)
• Kim MJ, Yang HJ, Kim Y et al. Role of nitric oxide and WNK-SPAK/OSR1-KCC2 signaling in daily changes in GABAergic inhibition in the rat dorsal raphe neurons. Neuropharmacology. Mar 27 2018 [PMID: 29596900] (Rat)
• Huguet L, Le Dudal M, Livrozet M et al. High frequency and wide range of human kidney papillary crystalline plugs. Urolithiasis 2017 Dec 12 [PMID: 29234857] (IHC, Human)
• Li J, Hatano R, Xu S et al. Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl-cotransporter activity and expression in male and female mice. Am. J. Physiol. Renal Physiol. Aug 1 2017 [PMID: 28566500] (WB, Mouse)
• Bhuiyan M, Song S, Yuan H et al. WNK-Cab39-NKCC1 signaling increases the susceptibility to ischemic brain damage in hypertensive rats. J Cereb Blood Flow Metab 2017 Jan 01 [PMID: 27798271] (WB, Rat)
• Roy A, Goodman J H et al. Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing. Am J Physiol Renal Physiol Feb 15 2015 [PMID: 25477473] (WB, Human)
• Andrukhova O, Smorodchenko A, Egerbacher M et al. FGF23 promotes renal calcium reabsorption through the TRPV5 channel. EMBO J. 2014 Feb 3 [PMID: 24434184] (WB, IP, IHC-P, EM, Mouse)
• Ohno M, Uchida K, Ohashi T et al. Immunolocalization of WNK4 in mouse kidney. Histochem Cell Biol. 2011 Jun 10. [PMID: 21660484] (WB, Mouse)
• Uchida S. Pathophysiological roles of WNK kinases in the kidney Pflugers Arch 2010 Sep [PMID: 20490538] (IHC, Mouse)
• Subramanya AR, Liu J, Ellison DH et al. WNK4 Diverts the Thiazide-sensitive NaCl Cotransporter to the Lysosome Stimulates AP-3 Interaction. J Biol Chem;284(27):18471-18480. 2009 [PMID: 19401467] (WB, Human, Mouse)
• Song, J et al. Regulation of blood pressure, the epithelial sodium channel (ENaC), and other key renal sodium transporters by chronic insulin infusion in rats. Am J Physiol Renal Physiol;290(5):F1055-64. 2006 May. [PMID: 16303859] (WB, Rat)
• Kahle, KT et al. WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia. Proc Natl Acad Sci U S A;101(7):2064-9. 2004 Feb 17. [PMID: 14769928] (WB, IHC-Fr, Human, Mouse)

Review for WNK4 Antibody (NB600-284) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Anonymous WB Rat 12/13/2017

Summary

• Application: Western Blot
• Sample Tested: Brain (cerebral cortex)
• Species: Rat
• Lot: B-2

Bioinformatics

• Gene Symbol: WNK4