WNK4 Antibody

Images

 
Western Blot: WNK4 Antibody [NB600-284] - Western Blot Image of anti-WNK4. Whole cell protein from Hek293 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, EM, IHC, IHC-Fr, IHC-P, IP, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

WNK4 Antibody Summary

Immunogen
A synthetic peptide made to a region of the human WNK4 protein between residues 200-300. [UniProt# Q96J92]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WNK4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Electron Microscopy
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
This WNK4 antibody is useful for Western blot where a band is seen at ~135-155 kDa. Immunohistochemistry-Frozen was reported in scientific literature. It is not useful for Immunocytochemistry/Immunofluorescence. Use in Electron Microscopy reported in scientific literature (PMID 24434184) Use in Immunohistochemistry-paraffin reported in scientific literature (PMID 24434184). Use in immunoprecipitation reported in scientific literature (PMID: 24434184).
Control
Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
WNK4 Peptide (NB600-284PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB600-284 in the following applications:

Publications
Read Publications using
NB600-284 in the following applications:

  • EM
    1 publication
  • IHC
    2 publications
  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    9 publications

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WNK4 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • PHA2B
  • PRKWNK4protein kinase, lysine deficient 4
  • Protein kinase lysine-deficient 4
  • Protein kinase with no lysine 4
  • serine/threonine-protein kinase WNK4
  • WNK lysine deficient protein kinase 4

Background

WNK4 (protein kinase with no lysine 4) is a novel serine/threonine kinase which plays critical role in regulation of electrolyte homeostasis, signal transduction, survival and proliferation. WNK4 is highly expressed in kidney, colon and skin where it forms a part of tight junction complex and interacts with/activate several proteins including SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5, NEDD4L and TRPV6. It control SLC12A3 (thiazide-sensitive Na-Cl cotransporter) activity by phosphorylation process that prevents membrane trafficking of SLC12A3. WNK4 inhibits KCNJ1 (renal K+ channel) via a kinase-independent action leading to induction of protein clearance from the cell surface by clathrin-dependent endocytosis. WNK4 acts as a molecular switch that controls NaCl reabsorption and K+ secretion for maintaining integrated homeostasis. Autophosphorylation of Ser-335 is essential for WNK4's activity whereas phosphorylation of Ser-331 also increase its activity. Mutations of WNK4 can cause pseudohypoaldosteronism type alpha (PHA alpha), an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-44270
Species: Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-P
NB600-225
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP1-48021
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-82874
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
H00009943-M16
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
NBP1-91725
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00130497-M10
Species: Hu, Mu
Applications: WB, ELISA, S-ELISA
NBP2-94269
Species: Hu, Mu, Rt
Applications: WB, IHC
NB100-75623
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB, IHC
NBP1-80993
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-67561
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P, IP
NBP1-87833
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-62346
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
NBP1-86892
Species: Hu
Applications: WB, IHC, IHC-P
NB300-562
Species: Hu, Mu, Rt, Ch, Rb, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, B/N
NBP2-33876
Species: Hu
Applications: WB, IHC, IHC-P
H00003930-B01P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45552
Species: Hu
Applications: WB, IHC
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
NB600-284
Species: Hu, Mu, Rt
Applications: WB, EM, IHC, IHC-Fr, IHC-P, IP, ICC/IF (-)

Publications for WNK4 Antibody (NB600-284)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: EM, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
EM
(1)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
IP
(1)
WB
(9)
All Applications
Filter By Species
Human
(4)
Mouse
(7)
Rat
(4)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB600-284 Applications Species
Zhang Y, Ye L, Yuan H, Duan D CFTR plays an important role in the regulation of vascular resistance and high-fructose/salt-diet induced hypertension in mice Journal of Cystic Fibrosis Dec 1 2020 [PMID: 33279469] (WB, Mouse) WB Mouse
Frame AA, Puleo F, Kim K et al. Sympathetic regulation of the NCC in norepinephrine-evoked salt-sensitive hypertension in Sprague-Dawley rats Am. J. Physiol. Renal Physiol. 2019 Oct 14 [PMID: 31608673] (Rat) Rat
Kim MJ, Yang HJ, Kim Y et al. Role of nitric oxide and WNK-SPAK/OSR1-KCC2 signaling in daily changes in GABAergic inhibition in the rat dorsal raphe neurons. Neuropharmacology. Mar 27 2018 [PMID: 29596900] (Rat) Rat
Huguet L, Le Dudal M, Livrozet M et al. High frequency and wide range of human kidney papillary crystalline plugs. Urolithiasis 2017 Dec 12 [PMID: 29234857] (IHC, Human) IHC Human
Li J, Hatano R, Xu S et al. Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl-cotransporter activity and expression in male and female mice. Am. J. Physiol. Renal Physiol. Aug 1 2017 [PMID: 28566500] (WB, Mouse) WB Mouse
Bhuiyan M, Song S, Yuan H et al. WNK-Cab39-NKCC1 signaling increases the susceptibility to ischemic brain damage in hypertensive rats. J Cereb Blood Flow Metab 2017 Jan 01 [PMID: 27798271] (WB, Rat) WB Rat
Roy A, Goodman J H et al. Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing. Am J Physiol Renal Physiol Feb 15 2015 [PMID: 25477473] (WB, Human) WB Human
Andrukhova O, Smorodchenko A, Egerbacher M et al. FGF23 promotes renal calcium reabsorption through the TRPV5 channel. EMBO J. 2014 Feb 3 [PMID: 24434184] (WB, IP, IHC-P, EM, Mouse) WB, IP, IHC-P, EM Mouse
Ohno M, Uchida K, Ohashi T et al. Immunolocalization of WNK4 in mouse kidney. Histochem Cell Biol. 2011 Jun 10. [PMID: 21660484] (WB, Mouse) WB Mouse
Uchida S. Pathophysiological roles of WNK kinases in the kidney Pflugers Arch 2010 Sep [PMID: 20490538] (IHC, Mouse) IHC Mouse
Show All 13 Publications.

Review for WNK4 Antibody (NB600-284) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB600-284:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Rat 12/13/2017
View

Summary

ApplicationWestern Blot
Sample TestedBrain (cerebral cortex)
SpeciesRat
LotB-2

Product General Protocols

View specific protocols for WNK4 Antibody (NB600-284): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for WNK4 Antibody (NB600-284) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-284AF350
Alexa Fluor 405 NB600-284AF405
Alexa Fluor 488 NB600-284AF488
Alexa Fluor 532 NB600-284AF532
Alexa Fluor 594 NB600-284AF594
Alexa Fluor 647 NB600-284AF647
Alexa Fluor 700 NB600-284AF700
Alexa Fluor 750 NB600-284AF750
Biotin NB600-284B
DyLight 350 NB600-284UV
DyLight 405 NB600-284V
DyLight 488 NB600-284G
DyLight 550 NB600-284R
DyLight 594 NB600-284DL594
DyLight 650 NB600-284C
DyLight 680 NB600-284FR
DyLight 755 NB600-284IR
FITC NB600-284F
HRP NB600-284H
Janelia Fluor 549 NB600-284JF549
Janelia Fluor 646 NB600-284JF646

Additional WNK4 Products

Bioinformatics Tool for WNK4 Antibody (NB600-284)

Discover related pathways, diseases and genes to WNK4 Antibody (NB600-284). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for WNK4 Antibody (NB600-284)

Discover more about diseases related to WNK4 Antibody (NB600-284).
 

Pathways for WNK4 Antibody (NB600-284)

View related products by pathway.

PTMs for WNK4 Antibody (NB600-284)

Learn more about PTMs related to WNK4 Antibody (NB600-284).
 

Research Areas for WNK4 Antibody (NB600-284)

Find related products by research area.

Blogs on WNK4

There are no specific blogs for WNK4, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
12/13/2017
Application: WB
Species: Rat

Bioinformatics

Gene Symbol WNK4