SLC12A3 Antibody

Western Blot: SLC12A3 Antibody [NBP1-44270] - Western blot analysis of Rat tissue lysates showing detection of SLC12A3 protein using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:1000.

Immunohistochemistry: SLC12A3 Antibody [NBP1-44270] - Immunohistochemistry analysis using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ICC/IF, IHC, MS
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SLC12A3 Antibody Summary

• Immunogen: Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
• Localization: Membrane
• Specificity: Detects approx 160kDa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC12A3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of NCC Antibody was sufficient for detection of NCC3 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31630621).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for SLC12A3 Antibody

• FLJ96318
• Na-Cl symporter
• NCC
• NCCT
• SLC12A3
• solute carrier family 12 (sodium/chloride transporters), member 3
• solute carrier family 12 member 3
• thiazide-sensitive Na-Cl cotransporter
• Thiazide-sensitive sodium-chloride cotransporter
• TSC
• TSCNaCl electroneutral thiazide-sensitive cotransporter

Background

NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Clreabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC12A3 Antibody (NBP1-44270)(1)

Publication using NBP1-44270

• Lu X, Rudemiller NP, Wen Y et al. A20 in Myeloid Cells Protects Against Hypertension by Inhibiting Dendritic Cell-Mediated T Cell Activation Circ. Res. 2019-10-21 [PMID: 31630621] (WB, Mouse)

Bioinformatics

• Gene Symbol: SLC12A3
• Entrez:
  • 6559(Human)
• Uniprot:
  • NP_062218(Rat)