SLC12A3 Antibody

Images

 
Western Blot: SLC12A3 Antibody [NBP1-44270] - analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:1000.
Immunocytochemistry/ Immunofluorescence: SLC12A3 Antibody [NBP1-44270] - Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit ...read more
Immunocytochemistry/ Immunofluorescence: SLC12A3 Antibody [NBP1-44270] - analysis of NCC in rat kidney tissues, showing a 1:200 dilution of this antibody

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, EM, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SLC12A3 Antibody Summary

Immunogen
Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
Localization
Membrane
Specificity
Detects approx 160kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC12A3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Electron Microscopy 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
1 ug/ml of NCC Antibody was sufficient for detection of NCC3 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.
Control
SLC12A3 Knockout HeLa Cell Lysate
Publications
Read Publication using
NBP1-44270 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31630621).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for SLC12A3 Antibody

  • FLJ96318
  • Na-Cl symporter
  • NCC
  • NCCT
  • SLC12A3
  • solute carrier family 12 (sodium/chloride transporters), member 3
  • solute carrier family 12 member 3
  • thiazide-sensitive Na-Cl cotransporter
  • Thiazide-sensitive sodium-chloride cotransporter
  • TSC
  • TSCNaCl electroneutral thiazide-sensitive cotransporter

Background

NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Clreabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC12A3 Antibody (NBP1-44270)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SLC12A3
Entrez
Uniprot