WNK4 Antibody [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

WNK4 Antibody [DyLight 405] Summary

Immunogen
A synthetic peptide made to a region of the human WNK4 protein between residues 200-300. [UniProt# Q96J92]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WNK4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunohistochemistry
Application Notes
This WNK4 antibody is useful for Western blot where a band is seen at ~135-155 kDa.

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for WNK4 Antibody [DyLight 405]

  • EC 2.7.11
  • EC 2.7.11.1
  • PHA2B
  • PRKWNK4protein kinase, lysine deficient 4
  • Protein kinase lysine-deficient 4
  • Protein kinase with no lysine 4
  • serine/threonine-protein kinase WNK4
  • WNK lysine deficient protein kinase 4

Background

WNK4 (protein kinase with no lysine 4) is a novel serine/threonine kinase which plays critical role in regulation of electrolyte homeostasis, signal transduction, survival and proliferation. WNK4 is highly expressed in kidney, colon and skin where it forms a part of tight junction complex and interacts with/activate several proteins including SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5, NEDD4L and TRPV6. It control SLC12A3 (thiazide-sensitive Na-Cl cotransporter) activity by phosphorylation process that prevents membrane trafficking of SLC12A3. WNK4 inhibits KCNJ1 (renal K+ channel) via a kinase-independent action leading to induction of protein clearance from the cell surface by clathrin-dependent endocytosis. WNK4 acts as a molecular switch that controls NaCl reabsorption and K+ secretion for maintaining integrated homeostasis. Autophosphorylation of Ser-335 is essential for WNK4's activity whereas phosphorylation of Ser-331 also increase its activity. Mutations of WNK4 can cause pseudohypoaldosteronism type alpha (PHA alpha), an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol WNK4