NKCC1/SLC12A2 Antibody

Images

 
Immunohistochemistry-Paraffin: NKCC1/SLC12A2 Antibody [NBP1-52148] - (3.8ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

NKCC1/SLC12A2 Antibody Summary

Immunogen
Peptide with sequence C-NALQHSIRLSGVEDH corresponding to internal region according to NP_001037.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SLC12A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave an approx. 75 kDa band in human, mouse and rat skeletal muscle, heart and brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 131 kDa band according to NP_001037.1. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human colon shows membranous staining.
Publications
Read Publication using NBP1-52148.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NKCC1/SLC12A2 Antibody

  • Basolateral Na-K-Cl symporter
  • BSC
  • BSC2
  • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
  • NKCC1
  • NKCC1MGC104233
  • SLC12A2
  • solute carrier family 12 (sodium/potassium/chloride transporters), member 2
  • solute carrier family 12 member 2

Background

NKCC1 (Na

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKCC1/SLC12A2 Antibody (NBP1-52148)(1)

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SLC12A2
Entrez
Uniprot