STK39 Antibody (OTI4E3)

Images

 
Western Blot: STK39 Antibody (4E3) [NBP1-48021] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-STK39 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: STK39 Antibody (4E3) [NBP1-48021] - Immunofluorescent staining of COS7 cells using anti-STK39 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Human Ovary tissue using anti-STK39 mouse monoclonal antibody.
Flow Cytometry: STK39 Antibody (4E3) [NBP1-48021] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-STK39 antibody, and then analyzed ...read more
Western Blot: STK39 Antibody (4E3) [NBP1-48021] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK39 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-STK39 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STK39 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Human endometrium tissue using anti-STK39 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI4E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

STK39 Antibody (OTI4E3) Summary

Immunogen
Full length human recombinant protein of human STK39 (NP_037365) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens serine threonine kinase 39 (STE20/SPS1 homolog, yeast) (STK39).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
STK39
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
59.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for STK39 Antibody (OTI4E3)

  • DCHTsmall intestine SPAK-like kinase
  • DKFZp686K05124
  • EC 2.7.11
  • EC 2.7.11.1
  • PASK
  • proline-alanine-rich STE20-related kinase
  • serine threonine kinase 39
  • Serine/threonine-protein kinase 39
  • SPAKserine threonine kinase 39 (STE20/SPS1 homolog, yeast)
  • STE20/SPS1 homolog
  • STE20/SPS1-related proline-alanine-rich protein kinase
  • Ste20-like protein kinase
  • Ste-20-related kinase

Background

This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STK39 Antibody (NBP1-48021) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-74406AF350
Alexa Fluor 405 NBP2-74406AF405
Alexa Fluor 488 NBP2-74406AF488
Alexa Fluor 532 NBP2-74406AF532
Alexa Fluor 594 NBP2-74406AF594
Alexa Fluor 647 NBP2-74406AF647
Alexa Fluor 700 NBP2-74406AF700
Alexa Fluor 750 NBP2-74406AF750
Allophycocyanin NBP2-74406APC
Allophycocyanin/Cy7 NBP2-74406APCCY7
Biotin NBP2-74406B
DyLight 350 NBP2-74406UV
DyLight 405 NBP2-74406V
DyLight 488 NBP2-74406G
DyLight 550 NBP2-74406R
DyLight 594 NBP2-74406DL594
DyLight 650 NBP2-74406C
DyLight 680 NBP2-74406FR
DyLight 755 NBP2-74406IR
FITC NBP2-74406F
HRP NBP2-74406H
Janelia Fluor 549 NBP2-74406JF549
Janelia Fluor 646 NBP2-74406JF646
PE NBP2-74406PE
PE/Atto594 NBP2-74406PEATT594
PE/Cy5.5 NBP2-74406PECY55
PE/Cy7 NBP2-74406PECY7
PerCP NBP2-74406PCP

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Bioinformatics

Gene Symbol STK39
Entrez