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STK39 Antibody (OTI4E3)

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Western Blot: STK39 Antibody (4E3) [NBP1-48021] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-STK39 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: STK39 Antibody (4E3) [NBP1-48021] - Immunofluorescent staining of COS7 cells using anti-STK39 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Human Ovary tissue using anti-STK39 mouse monoclonal antibody.
Flow Cytometry: STK39 Antibody (4E3) [NBP1-48021] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-STK39 antibody, and then analyzed ...read more
Western Blot: STK39 Antibody (4E3) [NBP1-48021] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK39 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-STK39 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STK39 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Human endometrium tissue using anti-STK39 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI4E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

STK39 Antibody (OTI4E3) Summary

Immunogen
Full length human recombinant protein of human STK39 (NP_037365) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens serine threonine kinase 39 (STE20/SPS1 homolog, yeast) (STK39).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
STK39
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500-1000
Theoretical MW
59.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-48021.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for STK39 Antibody (OTI4E3)

  • DCHTsmall intestine SPAK-like kinase
  • DKFZp686K05124
  • EC 2.7.11
  • EC 2.7.11.1
  • PASK
  • proline-alanine-rich STE20-related kinase
  • serine threonine kinase 39
  • Serine/threonine-protein kinase 39
  • SPAKserine threonine kinase 39 (STE20/SPS1 homolog, yeast)
  • STE20/SPS1 homolog
  • STE20/SPS1-related proline-alanine-rich protein kinase
  • Ste20-like protein kinase
  • Ste-20-related kinase

Background

This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STK39 Antibody (NBP1-48021)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STK39
Entrez