TRP2 Antibody

Western Blot: TRP2 Antibody [NBP1-86892] - Analysis in control (vector only transfected HEK293T lysate) and DCT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86892] - Staining of human testis.

Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86892] - Staining of human skin shows strong cytoplasmic positivity in melanocytes.

Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86892] - Staining of human skeletal muscle shows low expression as expected.

Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86892] - Staining in human skin and skeletal muscle tissues using anti-DCT antibody. Corresponding DCT RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86892] - Staining of human cerebral cortex, lymph node, skin and testis using Anti-DCT antibody NBP1-86892 (A) shows similar protein distribution across tissues to independent antibody NBP1-86893 (B).

Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86892] - Staining of human lymph node.

Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86892] - Staining of human cerebral cortex.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TRP2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNALEGFDKADGTLDSQVMSLHNLVHSFLNGTNALPHSAANDPIFVVLHSFTDA
• Specificity: Specificity of human TRP2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DCT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TRP2 Antibody

• dopachrome delta-isomerase
• dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
• DT
• EC 5.3.3.12
• L-dopachrome tautomerase
• TRP2
• TRP-2
• Tyrosinase-related protein 2
• TYRP2
• TYRP2L-dopachrome Delta-isomerase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRP2 Antibody (NBP1-86892)(1)

Publication using NBP1-86892

• Ponten F, Jirstrom K, Uhlen M et al. The Human Protein Atlas--a tool for pathology. J Pathol 2008 Dec [PMID: 18853439]

Bioinformatics

• Gene Symbol: DCT