Wee1 Antibody

Images

 
Western Blot: Wee1 Antibody [NBP1-33506] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with WEE1 antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody ...read more
Immunohistochemistry-Paraffin: Wee1 Antibody [NBP1-33506] - Paraffin-embedded CL1-0 Xenograft, using antibody at 1:100 dilution.
Western Blot: Wee1 Antibody [NBP1-33506] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal diluted at 1:500. The HRP-conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.95 mg/ml
 

Independent Antibodies

       

Order Details

Wee1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human WEE1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Rat (96%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WEE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (84%), Chicken (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
1XPBS, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Concentration
0.95 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Wee1 Antibody

  • DKFZp686I18166
  • EC 2.7.10.2
  • FLJ16446
  • WEE1 homolog (S. pombe)
  • wee1+ (S. pombe) homolog
  • WEE1+ homolog
  • Wee1A kinase
  • WEE1A
  • WEE1hu
  • wee1-like protein kinase

Background

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Wee1 Antibody (NBP1-33506)

Discover related pathways, diseases and genes to Wee1 Antibody (NBP1-33506). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Wee1 Antibody (NBP1-33506)

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Research Areas for Wee1 Antibody (NBP1-33506)

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Blogs on Wee1.

Funny Protein Names Infographic
This is not a joke, these proteins with funny names actually do exist. View our list of six proteins with funny and unusual names including: Bambi, Yippee-like 3, Wee1, SPAM1, SPOCK1 and Bagpipe homeobox protein homolog 1. Learn more about their fu...  Read full blog post.

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Bioinformatics

Gene Symbol WEE1