WDHD1 Antibody

Images

 
Western Blot: WDHD1 Antibody [NBP1-89091] - Analysis in human cell line RH-30.
Immunocytochemistry/ Immunofluorescence: WDHD1 Antibody [NBP1-89091] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human testis shows nuclear positivity in cells in seminiferous ducts.
Western Blot: WDHD1 Antibody [NBP1-89091] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431
Western Blot: WDHD1 Antibody [NBP1-89091] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Orthogonal Strategies: Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining in human testis and liver tissues.. Corresponding WDHD1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human liver shows no positivity in hepatocytes.
Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

WDHD1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QTLNIVTWSPCGQYLAAGSINGLIIVWNVETKDCMERVKHEKGYAICGLAWHPTCGRISYTDAEGNLGLLENVCDPSGKTSSSKVSSRVEKDYNDLFDGDDMSNAGDFLNDNAVE
Specificity
Specificity of human, mouse, rat WDHD1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WDHD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
126 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
WDHD1 Protein (NBP1-89091PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-89091 in the following applications:

Publications
Read Publications using
NBP1-89091 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for WDHD1 Antibody

  • Acidic nucleoplasmic DNA-binding protein 1
  • AND1
  • AND-1
  • CHTF4
  • CTF4
  • CTF4, chromosome transmission fidelity factor 4 homolog
  • WD repeat and HMG-box DNA binding protein 1
  • WD repeat and HMG-box DNA-binding protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WDHD1 Antibody (NBP1-89091)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Filter By Species
Human
(1)
All Species

Review for WDHD1 Antibody (NBP1-89091) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-89091:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot WDHD1 NBP1-89091
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reviewed by:
Anonymous
WB Human 11/11/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates
SpeciesHuman
Lot89091

Blocking

Blocking Details5% Milk

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-rabbit HRP
Secondary Concentration1:1000

Details

Detection NotesDensitometry for 30 seconds

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for WDHD1 Antibody (NBP1-89091) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for WDHD1 Antibody (NBP1-89091)

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Diseases for WDHD1 Antibody (NBP1-89091)

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PTMs for WDHD1 Antibody (NBP1-89091)

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Research Areas for WDHD1 Antibody (NBP1-89091)

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Blogs on WDHD1

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Recent Reviews

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1
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Anonymous
11/11/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol WDHD1
Entrez
Uniprot