Rad21 Antibody

Images

 
Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RAD21 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: Rad21 Antibody [NBP2-20053] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAD21 protein stained by RAD21 antibody diluted at 1:1000. Red: alpha Tubulin, ...read more
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Paraffin-embedded human lung papillary adenocarcinoma. RAD21 antibody diluted at 1:250.
Western Blot: Rad21 Antibody [NBP2-20053] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:3000.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:750 dilution.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:750 dilution.
Immunoprecipitation: Rad21 Antibody [NBP2-20053] - Jurkat whole cell extracts using 5 ug of RAD21 antibody. Western blot analysis was performed using RAD21 antibody. EasyBlot anti-Rabbit IgG was used as a secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Pm, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Rad21 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RAD21. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Rat (98%), Chicken (94%), Xenopus (90%), Primate (100%), Rhesus Macaque (100%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAD21
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-20053 in the following applications:

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (90%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Rad21 Antibody

  • double-strand-break repair protein rad21 homolog
  • hHR21FLJ25655
  • HR21
  • KIAA0078RAD21 (S. pombe) homolog
  • MCD1
  • Nuclear matrix protein 1
  • NXP-1
  • NXP1HRAD21
  • protein involved in DNA double-strand break repair
  • RAD21 homolog (S. pombe)
  • SCC1 homolog
  • SCC1FLJ40596

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-207
Species: Hu, Mu, Ce
Applications: WB, IHC, IHC-P, IP
NB100-330
Species: Hu
Applications: ICC/IF
NB100-204
Species: Hu, Mu
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-87145
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-75659
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB100-330
Species: Hu
Applications: ICC/IF
NB100-300
Species: Hu, Mu, Xp
Applications: WB, ICC/IF, ICC
H00025836-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-87957
Species: Hu
Applications: IHC, IHC-P
AF6820
Species: Mu, Rt
Applications: WB, IHC
NBP1-87097
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84379
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56494
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB
NBP1-87904
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-44687
Species: Hu, Mu
Applications: Flow, IHC, IHC-P, IF
NB100-563
Species: Hu
Applications: WB, IHC, IHC-P, IP
AF1095
Species: Hu
Applications: WB, Simple Western, IHC, CyTOF-ready, ICC, ICFlow
NBP2-20053
Species: Hu, Mu, Rt, Bv, Ch, Pm, RM, Xp
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for Rad21 Antibody (NBP2-20053) (0)

There are no publications for Rad21 Antibody (NBP2-20053).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for Rad21 Antibody (NBP2-20053) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-20053:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 04/11/2019
View

Summary

ApplicationWestern Blot
Sample Testedwhole cell lysate, Sample Amount: 40 ug
SpeciesHuman
Lot43110

Comments

CommentsWestern blot analysis of meningioma primary cell lysates yielded clear bands at the predicted molecular weight.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rad21 Antibody (NBP2-20053) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Rad21 Products

Bioinformatics Tool for Rad21 Antibody (NBP2-20053)

Discover related pathways, diseases and genes to Rad21 Antibody (NBP2-20053). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Rad21 Antibody (NBP2-20053)

Discover more about diseases related to Rad21 Antibody (NBP2-20053).
 

Pathways for Rad21 Antibody (NBP2-20053)

View related products by pathway.

PTMs for Rad21 Antibody (NBP2-20053)

Learn more about PTMs related to Rad21 Antibody (NBP2-20053).
 

Research Areas for Rad21 Antibody (NBP2-20053)

Find related products by research area.

Blogs on Rad21

There are no specific blogs for Rad21, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
04/11/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol RAD21
Uniprot