Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RAD21 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: Rad21 Antibody [NBP2-20053] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAD21 protein stained by RAD21 antibody diluted at 1:1000. Red: alpha Tubulin, ...read more
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Paraffin-embedded human lung papillary adenocarcinoma. RAD21 antibody diluted at 1:250.
Western Blot: Rad21 Antibody [NBP2-20053] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:3000.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:750 dilution.
Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:750 dilution.
Immunoprecipitation: Rad21 Antibody [NBP2-20053] - Jurkat whole cell extracts using 5 ug of RAD21 antibody. Western blot analysis was performed using RAD21 antibody. EasyBlot anti-Rabbit IgG was used as a secondary ...read more
72 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP2-20053 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 1% BSA, 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for Rad21 Antibody
double-strand-break repair protein rad21 homolog
KIAA0078RAD21 (S. pombe) homolog
Nuclear matrix protein 1
protein involved in DNA double-strand break repair
RAD21 homolog (S. pombe)
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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