CHL-1/L1CAM-2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse brain stem tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CHL‑1/L1CAM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2147) followed by ...read more
Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse brain stem tissue, loaded at 0.2 mg/mL. A specific band was detected for CHL‑1/L1CAM‑2 at approximately 196-201 kDa (as indicated) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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CHL-1/L1CAM-2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CHL‑1/L1CAM‑2
Ala25-Gln1043 (Leu227-Gln242 del, Ala243Ser)
Accession # BAC30699
Specificity
Detects mouse CHL‑1/L1CAM‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human CHL-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2147 in the following applications:

Publications
Read Publications using
AF2147 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CHL-1/L1CAM-2 Antibody [Unconjugated]

  • CALL
  • CALLClose homolog of L1
  • cell adhesion molecule with homology to L1CAM (close homolog of L1)
  • cell adhesion molecule with homology to L1CAM (close homologue of L1)
  • CHL1
  • CHL-1
  • FLJ44930
  • L1CAM-2
  • L1CAM2L1 cell adhesion molecule 2
  • MGC132578
  • neural cell adhesion molecule L1-like protein

Background

Close homolog of L1 (CHL-1), also known as cell adhesion L1-like (CALL) and L1 cell adhesion molecule 2 (L1CAM-2), belongs to the L1 subfamily of immunoglobulin (Ig) superfamily cell adhesion molecules, which also includes L1, neurofascin and NgCAM-related cell adhesion molecule (NrCAM) (1‑3). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4‑5 fibronectin type III-like (FNIII) domains in their extracellular regions. They also share a highly conserved cytoplasmic region of approximately 110 amino acid (aa) residues containing an ankyrin-binding site. CHL-1 is expressed as a highly glycosylated 185 kDa transmembrane protein by subpopulations of neurons and glia of the central and peripheral nervous system (4, 5). Ectodomain shedding via the metalloprotease-disintegrin ADAM8 releases 165 kDa and 125 kDa soluble CHL-1 fragments, which can diffuse away to function at distant sites (6). CHL-1 is not capable of homotypic interactions, but an extracellular binding partner of CHL-1 has not been identified (4). Human CHL1 has been mapped to chromosome 3p26 and is a candidate gene for 3p- syndrome characterized by mental impairment (7). A missense CHL1 polymorphism associated increased risk of schizophrenia, has also been reported (8). The functional importance of CHL-1 in the nervous system is also evident in CHL-1 deficient mice, which display behavioral abnormalities and show misguided axons within the hippocampus and olfactory tract (9). Enhanced ectodomain-shedding of CHL-1 is also observed in Wobbler mice, the neurodegenerative mutant mice (6). In vitro, soluble or substrate-coated CHL-1 promotes neurite outgrowth and neuronal survival of both cerebellar and hippocampal neurons. Cell surface CHL-1 interacts with integrins in cis to potentiate integrin-dependent cell migration toward extracellular matrix proteins (10). For this enhanced cell motility, CHL-1 linkage to the actin cytoskeleton via interaction between ankyrin and the CHL-1 cytoplasmic region is required.

  1. Moos, M. et al. (1988) Nature 334:701.
  2. Holm, J. et al. (1996) Eur. J. Neusci. 8:1613.
  3. Wei, M. et al. (1998) Hum. Genet. 103:355.
  4. Hillenbrand, R. et al. (1999) Eur. J. Neurosci. 11:813.
  5. Liu, Q. et al. (2000) J. Neurosci. 20:7682.
  6. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
  7. Angeloni, D. et al. (1999) Am. J. Med. Genet. 86:482.
  8. Sakurai, K. et al. (2002) Mol. Psychiatry 7:412.
  9. Montag-Sallaz, M. et al. (2002) Mol. Cell. Biol. 22:7967.
  10. Buhusi, M. et al. (2003) J. Biol. Chem. 278(27):25024.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHL-1/L1CAM-2 Antibody (AF2147)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Cell Culture, ICC, IHC, IHC-Fr, Immunoprecipitation, Western Blot.


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Cell Culture
(1)
ICC
(1)
IHC
(1)
IHC-Fr
(2)
Immunoprecipitation
(1)
Western Blot
(5)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF2147 Applications Species
S Lombardo, M Chiacchiar, A Tarr, W Kim, T Cao, G Sigal, TW Rosahl, W Xia, PG Haydon, ME Kennedy, G Tesco BACE1 partial deletion induces synaptic plasticity deficit in adult mice Sci Rep, 2019;9(1):19877. 2019 [PMID: 31882662] (Western Blot, Mouse) Western Blot Mouse
CR Bye, V Rytova, WF Alsanie, CL Parish, LH Thompson Axonal growth of midbrain dopamine neurons is modulated by the cell adhesion molecule ALCAM through trans-heterophilic interaction with L1Cam, ChL1 and Semaphorin J. Neurosci., 2019;0(0):. 2019 [PMID: 31300520] (Cell Culture, Mouse) Cell Culture Mouse
W Kim, L Ma, S Lomoio, R Willen, S Lombardo, J Dong, PG Haydon, G Tesco BACE1 elevation engendered by GGA3 deletion increases ?-amyloid pathology in association with APP elevation and decreased CHL1 processing in 5XFAD mice Mol Neurodegener, 2018;13(1):6. 2018 [PMID: 29391027] (Western Blot, Mouse) Western Blot Mouse
Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors. Proc Natl Acad Sci U S A, 2015;112(15):E1946-55. 2015 [PMID: 25775569] (IHC, Mouse) IHC Mouse
Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease. EMBO Mol Med, 2015;7(2):175-89. 2015 [PMID: 25592972] (Western Blot, Mouse) Western Blot Mouse
Hitt B, Riordan S, Kukreja L, Eimer W, Rajapaksha T, Vassar R beta-Site amyloid precursor protein (APP)-cleaving enzyme 1 (BACE1)-deficient mice exhibit a close homolog of L1 (CHL1) loss-of-function phenotype involving axon guidance defects. J Biol Chem, 2012;287(46):38408-25. 2012 [PMID: 22988240] (IHC-Fr, Mouse) IHC-Fr Mouse
Kuhn PH, Koroniak K, Hogl S, Colombo A, Zeitschel U, Willem M, Volbracht C, Schepers U, Imhof A, Hoffmeister A, Haass C, Rossner S, Brase S, Lichtenthaler SF Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons. EMBO J., 2012;31(14):3157-68. 2012 [PMID: 22728825] (Western Blot, Mouse) Western Blot Mouse
EphB regulates L1 phosphorylation during retinocollicular mapping. Mol. Cell. Neurosci., 2012;50(2):201-10. 2012 [PMID: 22579729] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Huang X, Zhu LL, Zhao T CHL1 negatively regulates the proliferation and neuronal differentiation of neural progenitor cells through activation of the ERK1/2 MAPK pathway. Mol. Cell. Neurosci., 2011;46(1):296-307. 2011 [PMID: 20933598] (IHC-Fr, Mouse) IHC-Fr Mouse
Wu J, Wrathall JR, Schachner M Phosphatidylinositol 3-kinase/protein kinase Cdelta activation induces close homolog of adhesion molecule L1 (CHL1) expression in cultured astrocytes. Glia, 2010;58(3):315-28. 2010 [PMID: 19672967] (Western Blot, Mouse) Western Blot Mouse
Show All 11 Publications.

Review for CHL-1/L1CAM-2 Antibody (AF2147) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2147:
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WB
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Mouse 01/06/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22579729
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22579729

Details

Detection NotesSee PMID 22579729

Comments

CommentsPublished: See PMID 22579729

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Biotin BAF2147

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Anonymous
01/06/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CHL1
Uniprot