MCM2 Antibody

Images

 
Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis ...read more
Immunohistochemistry: MCM2 Antibody [NB100-288] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Independent Antibodies: Immunoprecipitation: MCM2 Antibody [NB100-288] - Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from ...read more

Product Details

Summary
Reactivity Hu, XpSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

MCM2 Antibody Summary

Immunogen
A 37-amino acid synthetic peptide, which represented a portion of human Minichromosome Maintenance Deficient 2 encoded within exon 2 (LocusLink ID 4171).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:2000-1:10000
  • Immunoprecipitation 2-5 ug//mg lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-288 in the following applications:

Publications
Read Publications using
NB100-288 in the following applications:

  • IHC
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MCM2 Antibody

  • BM28
  • BM28MGC10606
  • CCNL 1
  • CCNL1
  • cdc19
  • CDCL1
  • CDCL1mitotin
  • D3S3194
  • EC 3.6.4.12
  • KIAA0030MITOTIN
  • MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
  • MCM2
  • minichromosome maintenance complex component 2
  • minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
  • minichromosome maintenance deficient 2 (mitotin)
  • Mitotin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-288
Species: Hu, Xp
Applications: WB, IHC, IHC-P, IP

Publications for MCM2 Antibody (NB100-288)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(2)
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Filter By Species
Human
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-288 Applications Species
Hubbi ME, Gilkes DM, Hu H et al. Cyclin-dependent kinases regulate lysosomal degradation of hypoxia-inducible factor 1alpha to promote cell-cycle progression. Proc Natl Acad Sci U S A. 2014 Jul 28 [PMID: 25071185] (WB, Human) WB Human
Hubbi ME, Kshitiz, Gilkes DMet al. A nontranscriptional role for HIF-1alpha as a direct inhibitor of DNA replication. Sci Signal 2013 Feb 12 [PMID: 23405012]
Isaacs JT, D'Antonio JM, Chen S et al. Adaptive auto-regulation of androgen receptor provides a paradigm shifting rationale for bipolar androgen therapy (BAT) for castrate resistant human prostate cancer. Prostate 2012 Oct [PMID: 22396319]
Hubbi ME, Luo W, Baek JH et al. MCM proteins are negative regulators of hypoxia-inducible factor 1. Mol Cell. Jun. 2011 [PMID: 21658608] (WB, Human) WB Human
Gueranger Q, Kia A, Frith D et al. Crosslinking of DNA repair and replication proteins to DNA in cells treated with 6-thioguanine and UVA. Nucleic Acids Res 2011 Jul [PMID: 21398635]
Kadara H, Behrens C, Yuan P et al. A five-gene and corresponding protein signature for stage-I lung adenocarcinoma prognosis Clin Cancer Res 2011 Mar 15 [PMID: 21163870] (IHC, Human) IHC Human
Snyder M, Huang XY, Zhang JJ et al. The minichromosome maintenance proteins 2-7 (MCM2-7) are necessary for RNA polymerase II (Pol II)-mediated transcription. J Biol Chem 2009 May [PMID: 19318354]
Pacek M, Tutter AV, Kubota Y et al. Localization of MCM2-7, Cdc45, and GINS to the site of DNA unwinding during eukaryotic DNA replication. Mol Cell 2006 Feb [PMID: 16483939]

Reviews for MCM2 Antibody (NB100-288) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-288:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 08/10/2010
View

Summary

ApplicationWestern Blot
Sample Tested293T Western Cell lysate
SpeciesHuman
CommentsClear distinct band by western blot.

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:2000, Incubation Dilution Buffer: 5% in PBST, Incubation Time: overnight, Incubation Temp: 4 degrees Celsius

Comments

CommentsClear distinct band by western blot.
 
reviewed by:
Anonymous
WB Human 01/26/2010
View

Summary

ApplicationWestern Blot
Sample TestedHCT116 Cell Lysate, Sample Amount: 50 ug
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk In PBS-T, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/2000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Details

Detection NotesPositive Control: HCT116 Cell Lysate, Specific Bands: 100 kDa

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MCM2 Antibody (NB100-288) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MCM2 Antibody (NB100-288)

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Recent Reviews

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5
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1
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Anonymous
08/10/2010
Application: WB
Species: Human

 
Anonymous
01/26/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol MCM2
Entrez
Uniprot