MCM2 Antibody

Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry: MCM2 Antibody [NB100-288] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: MCM2 Antibody [NB100-288] - Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for IP at 3 ug per reaction. MCM2 was also immunoprecipitated by rabbit anti-Phospho MCM2 (S108) antibody NB100-639. For blotting immunoprecipitated MCM2, NB100-288 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Xp
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

MCM2 Antibody Summary

• Immunogen: A 37-amino acid synthetic peptide, which represented a portion of human Minichromosome Maintenance Deficient 2 encoded within exon 2 (LocusLink ID 4171).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MCM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:2000-1:10000
  • Immunoprecipitation 2-5 ug//mg lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MCM2 Antibody

• BM28
• BM28MGC10606
• CCNL 1
• CCNL1
• cdc19
• CDCL1
• CDCL1mitotin
• D3S3194
• EC 3.6.4.12
• KIAA0030MITOTIN
• MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
• MCM2
• minichromosome maintenance complex component 2
• minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
• minichromosome maintenance deficient 2 (mitotin)
• Mitotin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCM2 Antibody (NB100-288)(8)

Publications using NB100-288

• Hubbi ME, Gilkes DM, Hu H et al. Cyclin-dependent kinases regulate lysosomal degradation of hypoxia-inducible factor 1alpha to promote cell-cycle progression. Proc Natl Acad Sci U S A. 2014 Jul 28 [PMID: 25071185] (WB, Human)
• Hubbi ME, Kshitiz, Gilkes DMet al. A nontranscriptional role for HIF-1alpha as a direct inhibitor of DNA replication. Sci Signal 2013 Feb 12 [PMID: 23405012]
• Isaacs JT, D'Antonio JM, Chen S et al. Adaptive auto-regulation of androgen receptor provides a paradigm shifting rationale for bipolar androgen therapy (BAT) for castrate resistant human prostate cancer. Prostate 2012 Oct [PMID: 22396319]
• Hubbi ME, Luo W, Baek JH et al. MCM proteins are negative regulators of hypoxia-inducible factor 1. Mol Cell. Jun. 2011 [PMID: 21658608] (WB, Human)
• Gueranger Q, Kia A, Frith D et al. Crosslinking of DNA repair and replication proteins to DNA in cells treated with 6-thioguanine and UVA. Nucleic Acids Res 2011 Jul [PMID: 21398635]
• Kadara H, Behrens C, Yuan P et al. A five-gene and corresponding protein signature for stage-I lung adenocarcinoma prognosis Clin Cancer Res 2011 Mar 15 [PMID: 21163870] (IHC, Human)
• Snyder M, Huang XY, Zhang JJ et al. The minichromosome maintenance proteins 2-7 (MCM2-7) are necessary for RNA polymerase II (Pol II)-mediated transcription. J Biol Chem 2009 May [PMID: 19318354]
• Pacek M, Tutter AV, Kubota Y et al. Localization of MCM2-7, Cdc45, and GINS to the site of DNA unwinding during eukaryotic DNA replication. Mol Cell 2006 Feb [PMID: 16483939]

Reviews for MCM2 Antibody (NB100-288) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anonymous WB Human 08/10/2010

Summary

• Application: Western Blot
• Sample Tested: 293T Western Cell lysate
• Species: Human
• Comments: Clear distinct band by western blot.

Blocking

• Blocking Details: Blocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

• Dilution Ratio: Dilution Ratio: 1:2000, Incubation Dilution Buffer: 5% in PBST, Incubation Time: overnight, Incubation Temp: 4 degrees Celsius

Comments

• Comments: Clear distinct band by western blot.

reviewed by: Anonymous WB Human 01/26/2010

Summary

• Application: Western Blot
• Sample Tested: HCT116 Cell Lysate, Sample Amount: 50 ug
• Species: Human

Blocking

• Blocking Details: Blocking Buffer: 5% Milk In PBS-T, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1/2000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Details

• Detection Notes: Positive Control: HCT116 Cell Lysate, Specific Bands: 100 kDa

Bioinformatics

• Gene Symbol: MCM2
• Entrez:
  • 4171(Human)
• Uniprot:
  • P49736()