DDX11 Antibody (3C1) - Azide and BSA Free

Western Blot: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of DDX11 expression in human liver.

Immunocytochemistry/Immunofluorescence: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of monoclonal antibody to DDX11 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of monoclonal antibody to DDX11 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml

Western Blot: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of DDX11 expression in HeLa(Cat # L013V1).

Sandwich ELISA: DDX11 Antibody (3C1) [H00001663-M03] - Detection limit for recombinant GST tagged DDX11 is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DDX11 Antibody (3C1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DDX11 (AAH11264, 1 a.a. ~ 970 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANETQKVGAIHFPFPFTPYSIQEDFMAELYRVLEAGKIGIFESPTGTGKSLSLICGALSWLRDFEQKKREEEARLLETGTGPLHDEKDESLCLSSSCEGAAGTPRPAGEPAWVTQFVQKKEERDLVDRLKAEQARRKQREERLQQLQHRVQLKYAAKRLRQEEEERENLLRLSREMLETGPEAERLEQLESGEEELVLAEYESDEEKKVASRVDEDEDDLEEEHITKIYYCSRTHSQLAQFVHEVKKSPFGKDVRLVSLGSRQNLCVNEDVKSLGSVQLINDRCVDMQRSRHEKKKGAEEEKPKRRRQEKQAACPFYNHEQMGLLRDEALAEVKDMEQLLALGKEARACPYYGSRLAIPAAQLVVLPYQMLLHAATRQAAGIRLQDQVVIIDEAHNLIDTITGMHSVEVSGSQLCQAHSQLLQYVERYGKRLKAKNLMYLKQILYLLEKFVAVLGGNIKQNPNTQSLSQTGTELKTINDFLFQSQIDNINLFKVQRYCEKSMISRKLFGFTERYGAVFSSREQPKLAGFQQFLQSLQPRTTEALAAPADESQASTLRPASPLMHIQGFLAALTTANQDGRVILSRQGSLSQSTLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPVSDFRQQLLACAGVEAERVVEFSCGHVIPPDNILPLVICSGISNQPLEFTFQKRELPQMMDEVGRILCNLCGVVPGGVVCFFPSYEYLRQVHAHWEKGGLLGRLAARKKIFQEPKSAHQVEQVLLAYSRCIQACGQERGQVTGALLLSVVGGKMSEGINFSDNLGRCVVMVGMPFPNIRSAELQEKMAYLDQTLSPRPGTPREGSGGEPVHEGRQPVHRQGHQAPEGFCQRSAPGPAICPAPCPGQAAGLDPSPCGGQSYLWPRHCCCAEVSPGEVGLFLMGNHATAWRRALPLSCPLETVFVVGVVCGDPVTKVKPRRRVWSPECCQDPGTGVSSRRRKWGNPE
• Specificity: DDX11 (3C1)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDX11
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: It has been used for ELISA and WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DDX11 Antibody (3C1) - Azide and BSA Free

• CHL1CHL1-related helicase gene-1
• CHL1-related protein 1
• CHLR1MGC9335
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S.cerevisiae)
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (S.cerevisiae CHL1-likehelicase)
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11
• DEAD/H box protein 11
• EC 3.6.1
• EC 3.6.1.23
• EC 3.6.1.7
• EC 3.6.4.13
• hCHLR1
• Keratinocyte growth factor-regulated gene 2 protein
• KRG-2
• KRG2CHL1-like helicase homolog
• MGC133249
• probable ATP-dependent RNA helicase DDX11

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX11 Antibody (H00001663-M03)(1)

Publication using H00001663-M03

• Perez-Benavente B, Garcia JL, Rodriguez MS et al. GSK3-SCFFBXW7 targets JunB for degradation in G2 to preserve chromatid cohesion before anaphase Oncogene 2012-06-18 [PMID: 22710716] (WB, Human)

Bioinformatics

• Gene Symbol: DDX11
• Entrez:
  • 1663(Human)
• Uniprot:
  • AAH11264(Human)