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DDX11 Antibody (3C1)

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Western Blot: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of DDX11 expression in human liver.
Western Blot: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of DDX11 expression in human liver.
Immunocytochemistry/ Immunofluorescence: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of monoclonal antibody to DDX11 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of monoclonal antibody to DDX11 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml
Western Blot: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of DDX11 expression in HeLa(Cat # L013V1).
Sandwich ELISA: DDX11 Antibody (3C1) [H00001663-M03] - Detection limit for recombinant GST tagged DDX11 is 3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Clone
3C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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DDX11 Antibody (3C1) Summary

Immunogen
DDX11 (AAH11264, 1 a.a. ~ 970 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANETQKVGAIHFPFPFTPYSIQEDFMAELYRVLEAGKIGIFESPTGTGKSLSLICGALSWLRDFEQKKREEEARLLETGTGPLHDEKDESLCLSSSCEGAAGTPRPAGEPAWVTQFVQKKEERDLVDRLKAEQARRKQREERLQQLQHRVQLKYAAKRLRQEEEERENLLRLSREMLETGPEAERLEQLESGEEELVLAEYESDEEKKVASRVDEDEDDLEEEHITKIYYCSRTHSQLAQFVHEVKKSPFGKDVRLVSLGSRQNLCVNEDVKSLGSVQLINDRCVDMQRSRHEKKKGAEEEKPKRRRQEKQAACPFYNHEQMGLLRDEALAEVKDMEQLLALGKEARACPYYGSRLAIPAAQLVVLPYQMLLHAATRQAAGIRLQDQVVIIDEAHNLIDTITGMHSVEVSGSQLCQAHSQLLQYVERYGKRLKAKNLMYLKQILYLLEKFVAVLGGNIKQNPNTQSLSQTGTELKTINDFLFQSQIDNINLFKVQRYCEKSMISRKLFGFTERYGAVFSSREQPKLAGFQQFLQSLQPRTTEALAAPADESQASTLRPASPLMHIQGFLAALTTANQDGRVILSRQGSLSQSTLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPVSDFRQQLLACAGVEAERVVEFSCGHVIPPDNILPLVICSGISNQPLEFTFQKRELPQMMDEVGRILCNLCGVVPGGVVCFFPSYEYLRQVHAHWEKGGLLGRLAARKKIFQEPKSAHQVEQVLLAYSRCIQACGQERGQVTGALLLSVVGGKMSEGINFSDNLGRCVVMVGMPFPNIRSAELQEKMAYLDQTLSPRPGTPREGSGGEPVHEGRQPVHRQGHQAPEGFCQRSAPGPAICPAPCPGQAAGLDPSPCGGQSYLWPRHCCCAEVSPGEVGLFLMGNHATAWRRALPLSCPLETVFVVGVVCGDPVTKVKPRRRVWSPECCQDPGTGVSSRRRKWGNPE
Specificity
DDX11 (3C1)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DDX11
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
It has been used for ELISA and WB.
Publications
Read Publication using
H00001663-M03 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DDX11 Antibody (3C1)

  • CHL1CHL1-related helicase gene-1
  • CHL1-related protein 1
  • CHLR1MGC9335
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S.cerevisiae)
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (S.cerevisiae CHL1-likehelicase)
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11
  • DEAD/H box protein 11
  • EC 3.6.1
  • EC 3.6.1.23
  • EC 3.6.1.7
  • EC 3.6.4.13
  • hCHLR1
  • Keratinocyte growth factor-regulated gene 2 protein
  • KRG-2
  • KRG2CHL1-like helicase homolog
  • MGC133249
  • probable ATP-dependent RNA helicase DDX11

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: L1CAM Antibody (UJ127.11) [NB100-2682] - Total protein from human HeLa, A431 and SHSY-5Y cell lines and human Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-L1CAM (NB100-2682) in blocking buffer and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).
Immunohistochemistry-Paraffin: L1CAM Antibody (UJ127.11) [NB100-2682] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of L1CAM [UJ127.11] antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic and membrane staining as observed in the cerebral cortex
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Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Western Blot: CHTF18 Antibody (1F5) [H00063922-M01] - CHTF18 monoclonal antibody (M01), clone 1F5 Analysis of CHTF18 expression in HeLa.
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Transient Overexpress ...
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PP2A alpha [p
Tyr307] Antibody [Unc ...
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Publications for DDX11 Antibody (H00001663-M03)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol DDX11
Entrez
Uniprot