WAPL Antibody - BSA Free

Western Blot: WAPL Antibody [NB100-552] - Detection of human WAPL by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WAPL antibody NB100-552 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

WAPL Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit WAPL Antibody - BSA Free (NB100-552) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1125 and 1175 of human wings apart like/KIAA0261 using the numbering given in TrEMBL entry Q7Z5K2 (GeneID 23063).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WAPL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2500-1:10000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:2,500 - 1:10,000 Immunoprecipitation: not tested Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WAPL Antibody - BSA Free

• FOEKIAA0261friend of EBNA2 (Epstein-Barr virus nuclear protein 2)
• Friend of EBNA2 protein
• WAPLwings apart-like protein homolog
• wings apart-like homolog (Drosophila)

Background

The hWAPL mRNA was predominantly expressed in uterine cervical cancer, with weak expression in all other normal and tumor tissues examined. hWAPL expression in benign epithelia was confined to the basal cell layers, whereas in dysplasias it increasingly appeared in more superficial cell layers and showed a significant correlation with severity of dysplasia. Diffuse hWAPL expression was found in all invasive squamous cell carcinomas examined. These results demonstrate that hWAPL is associated with cell growth, and the hWAPL expression may play a significant role in cervical carcinogenesis and tumor progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WAPL
• Entrez:
  • 23063(Human)
• Uniprot:
  • Q7Z5K2(Human)