DEP-1/CD148 Antibody (143-41)

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human DEP‑1/CD148 Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP, CyTOF-ready
Clone
143-41
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

DEP-1/CD148 Antibody (143-41) Summary

Immunogen
Phytohemagglutinin-stimulated human peripheral blood mononuclear cells
Specificity
Detects human DEP-1/CD148 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTPRJ
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunoprecipitation 2.5 ug/500 ug cell lysate
  • CyTOF-ready
Reviewed Applications
Read 2 Reviews rated 4.5
using
MAB1934 in the following applications:

Publications
Read Publications using
MAB1934 in the following applications:

  • 2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DEP-1/CD148 Antibody (143-41)

  • CD148 antigen
  • CD148
  • Density-enhanced phosphatase 1
  • DEP1
  • DEP-1
  • DEP1EC 3.1.3.48
  • HPTP eta
  • HPTPeta
  • HPTP-eta
  • human density enhanced phosphatase-1
  • protein tyrosine phosphatase, receptor type, J polypeptide
  • protein tyrosine phosphatase, receptor type, J
  • Protein-tyrosine phosphatase eta
  • Protein-tyrosine phosphatase receptor type J
  • PTPRJ
  • receptor-type tyrosine-protein phosphatase eta
  • R-PTP-ETA
  • R-PTP-J
  • SCC1
  • susceptibility to colon cancer 1, mouse, homolog of

Background

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T‑cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

  1. Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
  2. Sorby, M. et al. (2001) Oncogene 20:5219.
  3. Jandt, E. et al. (2003) Oncogene 22:4175.
  4. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
  5. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
  6. Borges, L.G. et al. (1996) Circ. Res. 79:570.
  7. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
  8. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
  9. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
  10. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
  11. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
  12. Holsinger, L.J. et al. (2002) Oncogene 21:7067.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DEP-1/CD148 Antibody (MAB1934)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow, WB.


Filter By Application
Flow
(2)
WB
(2)
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Filter By Species
Human
(4)
All Species

Reviews for DEP-1/CD148 Antibody (MAB1934) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using MAB1934:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 02/12/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22564856
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22564856

Details

Detection NotesSee PMID 22564856

Comments

CommentsIndependent review from antibodyresource.comUsed this antibody for Western blotting and Flow Cytometry.
 
reviewed by:
Anonymous
WB Mouse 02/12/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22564856
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22564856

Details

Detection NotesSee PMID 22564856

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22564856

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
02/12/2015
Application: WB
Species: Human

 
Anonymous
02/12/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PTPRJ