SCC112 Antibody - BSA Free

Western Blot: SCC112 Antibody [NBP2-38313] - Analysis using Anti-PDS5A antibody NBP2-38313 (A) shows similar pattern to independent antibody NBP1-87904 (B).

Immunocytochemistry/Immunofluorescence: SCC112 Antibody [NBP2-38313] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: SCC112 Antibody [NBP2-38313] - Staining of human lymph node.

Immunohistochemistry: SCC112 Antibody [NBP2-38313] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: SCC112 Antibody [NBP2-38313] - Staining of human cerebral cortex, colon, kidney and lymph node using Anti-PDS5A antibody NBP2-38313 (A) shows similar protein distribution across tissues to independent antibody NBP1-87904 (B).

Immunohistochemistry-Paraffin: SCC112 Antibody [NBP2-38313] - Staining of human cerebral cortex.

Immunohistochemistry-Paraffin: SCC112 Antibody [NBP2-38313] - Staining of human kidney.

Immunohistochemistry-Paraffin: SCC112 Antibody [NBP2-38313] - Staining of human colon.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SCC112 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SCC112 Antibody - BSA Free (NBP2-38313) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: RSTGTETGSNINVNSELNPSTGNRSREQSSEAAETGVSENEENPVRIISVTPVKNIDPVKNKEINSDQATQGNISSDRGKKRTVTAA
• Predicted Species: Mouse (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDS5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SCC112 Protein (NBP2-38313PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SCC112 Antibody - BSA Free

• Cell proliferation-inducing gene 54 protein
• DKFZp686B19246
• FLJ41012
• KIAA0648MGC131948
• PDS5
• PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
• PIG54
• SCC-112MGC161503
• Sister chromatid cohesion protein 112
• sister chromatid cohesion protein PDS5 homolog A

Background

SCC-112 is a novel cell cycle-regulated molecule. It is a nuclear protein of ~147 kDa, suggested to be associated with normal cell growth and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDS5A
• Uniprot:
  • Q29RF7(Human)