Separase Antibody (6H6)

Images

 
Western Blot: Separase Antibody (6H6) [H00009700-M01] - ESPL1 monoclonal antibody (M01), clone 6H6 Analysis of ESPL1 expression in Hela S3 NE.
Immunohistochemistry-Paraffin: Separase Antibody (6H6) [H00009700-M01] - Analysis of monoclonal antibody to ESPL1 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
6H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Separase Antibody (6H6) Summary

Immunogen
ESPL1 (NP_036423, 586 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. REELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKIQEGIERDR
Specificity
ESPL1 - extra spindle poles like 1 (S. cerevisiae)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ESPL1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28859055).
Publications
Read Publications using
H00009700-M01 in the following applications:

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Separase Antibody (6H6)

  • Caspase-like protein ESPL1
  • EC 3.4.22.49
  • ESP1extra spindle poles like 1
  • extra spindle pole bodies homolog 1 (S. cerevisiae)
  • extra spindle poles like 1 (S. cerevisiae)
  • Extra spindle poles-like 1 protein
  • FLJ46492
  • KIAA0165separin
  • Separase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Separase Antibody (H00009700-M01)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using H00009700-M01 Applications Species
Gurvits N, Loyttyniemi E, Nykanen M et al. Separase is a marker for prognosis and mitotic activity in breast cancer. Br. J. Cancer. Oct 24 2017 [PMID: 28859055] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Mukherjee M, Byrd T, Brawley VS et al. Overexpression and constitutive nuclear localization of cohesin protease Separase protein correlates with high incidence of relapse and reduced overall survival in glioblastoma multiforme. J Neurooncol. 2014 May 04 [PMID: 24792645]
Zhang N, Scorsone K, Ge G et al. Identification and Characterization of Separase Inhibitors (Sepins) for Cancer Therapy. J Biomol Screen. 2014 Feb 19 [PMID: 24525869]
Mukherjee M, Ge G, Zhang N et al. MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ERalpha)-positive mammary adenocarcinomas. Oncogene. 2013 Nov 25 [PMID: 24276237]
Panigrahi AK, Zhang N, Mao Q, Pati D. Calpain-1 Cleaves Rad21 to Promote Sister Chromatid Separation. Mol Cell Biol. 2011 Aug 29 [PMID: 21876002]
Mukherjee M, Ge G, Zhang N et al. Separase Loss of Function Cooperates with the Loss of p53 in the Initiation and Progression of T- and B-Cell Lymphoma, Leukemia and Aneuploidy in Mice. PLoS One. 2011 Jul 25 [PMID: 21799785]
Zhang N, Ge G, Meyer R et al. Overexpression of Separase induces aneuploidy mammary tumorigenesis. Proc Natl Acad Sci U S A 105(35):13033-8. 2008 Sep 2. [PMID: 18728194]
Repo H SECURIN-RELATED CELL PROLIFERATION IN BREAST CANCER PROGNOSIS Thesis Jan 1 2020 (IHC-P, Human) IHC-P Human
Meyer R, Fofanov V, Panigrahi A et al. Overexpression Mislocalization of the Chromosomal Segregation Protein Separase in Multiple Human Cancers. Clin Cancer Res;15(8):2703-10. 2009 Apr 15. [PMID: 19351757]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ESPL1
Entrez
OMIM
Uniprot