Separase Antibody (6H6)

Images

 
Western Blot: Separase Antibody (6H6) [H00009700-M01] - ESPL1 monoclonal antibody (M01), clone 6H6 Analysis of ESPL1 expression in Hela S3 NE.
Immunohistochemistry-Paraffin: Separase Antibody (6H6) [H00009700-M01] - Analysis of monoclonal antibody to ESPL1 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
6H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Separase Antibody (6H6) Summary

Immunogen
ESPL1 (NP_036423, 586 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. REELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKIQEGIERDR
Specificity
ESPL1 - extra spindle poles like 1 (S. cerevisiae)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ESPL1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28859055).
Publications
Read Publications using
H00009700-M01 in the following applications:

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Separase Antibody (6H6)

  • Caspase-like protein ESPL1
  • EC 3.4.22.49
  • ESP1extra spindle poles like 1
  • extra spindle pole bodies homolog 1 (S. cerevisiae)
  • extra spindle poles like 1 (S. cerevisiae)
  • Extra spindle poles-like 1 protein
  • FLJ46492
  • KIAA0165separin
  • Separase

Background

Separase is a cysteine protease that is essential for mitotic progression by separating sister chromatids. Each cell must receive one chromatid of every chromosome during mitosis. Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys cohesin by means of cleavage, allowing the chromatids to separate and divide with the cell. Separase activity is highly regulated. It not only cleaves cohesin at the onset of anaphase, but also cleaves itself, promoting downregulation of separase after anaphase. Should a human cell become an aneuploid (one too many or too few chromatids), the embryo most likely will not survive. Should the embryo survive, it will most likely develop severe birth defects or later develop malignant cancers. Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Separase Antibody (H00009700-M01)(14)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IF/IHC, IHC-P.


Filter By Application
ICC/IF
(1)
IF/IHC
(1)
IHC-P
(1)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using H00009700-M01 Applications Species
Hiba O, Cameron G, Mathieu M et al. Mitogen-Induced Defective Mitosis Transforms Neural Progenitor Cells. Neuro Oncol. 2023-04-26 [PMID: 37186014]
H V, K K, T A et al. Varying outcomes of triple-negative breast cancer in different age groups-prognostic value of clinical features and proliferation. Breast Cancer Res Treat. 2022-10-19 [PMID: 36261751]
C Thomas, B Wetherall, MD Levasseur, RJ Harris, ST Kerridge, JMG Higgins, OR Davies, S Madgwick A prometaphase mechanism of securin destruction is essential for meiotic progression in mouse oocytes Nature Communications, 2021-07-14;12(1):4322. 2021-07-14 [PMID: 34262048]
Claudia G, Elena A, Maria U et al. Centrosome Reduction in Newly-Generated Tetraploid Cancer Cells Obtained by Separase Depletion. Sci Rep. 2020-06-04 [PMID: 32499568]
Vihervuori H, Autere T, Repo H et al. Tumor-infiltrating lymphocytes and CD8+ T cells predict survival of triple-negative breast cancer. J Cancer Res Clin Oncol. 2019-09-27 [PMID: 31562550]
Gurvits N, Loyttyniemi E, Nykanen M et al. Separase is a marker for prognosis and mitotic activity in breast cancer. Br. J. Cancer. 2017-10-24 [PMID: 28859055] (IF/IHC, ICC/IF, Human) IF/IHC, ICC/IF Human
Mukherjee M, Byrd T, Brawley VS et al. Overexpression and constitutive nuclear localization of cohesin protease Separase protein correlates with high incidence of relapse and reduced overall survival in glioblastoma multiforme. J Neurooncol. 2014-05-04 [PMID: 24792645]
Zhang N, Scorsone K, Ge G et al. Identification and Characterization of Separase Inhibitors (Sepins) for Cancer Therapy. J Biomol Screen. 2014-02-19 [PMID: 24525869]
Mukherjee M, Ge G, Zhang N et al. MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ERalpha)-positive mammary adenocarcinomas. Oncogene. 2013-11-25 [PMID: 24276237]
Panigrahi AK, Zhang N, Mao Q, Pati D. Calpain-1 Cleaves Rad21 to Promote Sister Chromatid Separation. Mol Cell Biol. 2011-08-29 [PMID: 21876002]
Show All 14 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ESPL1
Entrez
OMIM
Uniprot