VPS4B Antibody - BSA Free

Immunoprecipitation: VPS4B Antibody - BSA Free [NBP3-45587] - IP Result of anti-VPS4B (IP:, 4ug; Detection: 1:1000) with HeLa cells lysate 880ug.

Western Blot: VPS4B Antibody - BSA Free [NBP3-45587] - Y79 cells were subjected to SDS PAGE followed by western blot with (VPS4B antibody) at dilution of 1:500

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VPS4B Antibody - BSA Free Summary

• Immunogen: vacuolar protein sorting 4 homolog B. (Uniprot# O75351)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS4B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:100
  • Immunoprecipitation 1:500-1:5000
  • Western Blot 1:500-1:5000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for VPS4B Antibody - BSA Free

• Cell migration-inducing gene 1 protein
• Protein SKD1
• SKD1B
• SKD1cell migration-inducing 1
• Suppressor of K(+) transport growth defect 1
• suppressor of K+ transport defect 1
• vacuolar protein sorting 4 homolog B (S. cerevisiae)
• vacuolar protein sorting 4B (yeast)
• vacuolar protein sorting 4B
• vacuolar protein sorting-associated protein 4B
• VPS42
• VPS4-2

Background

VPS4B is encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VPS4B