MIG2/Kindlin-2 Antibody (OTI9E4)

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - GST-tagged wild-type MIG2/Kindlin-2 or MIG2/Kindlin-2 (S159A) were incubated without IKKE (-), or with purified wild-type IKKE (WT) or mutant IKKE (K38A) in kinase buffer and resolved by SDS-PAGE, followed by immunoblotting analysis with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC7019159/) licensed under a CC-BY license

Immunocytochemistry/Immunofluorescence: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of HepG2 cells using anti-Kindlin-2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human pancreas tissue using anti-Kindlin-2 mouse monoclonal antibody.

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kindlin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Kindlin-2.

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Kindlin-2 monoclonal antibody.

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human colon tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human Ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunoprecipitation: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, ICC/IF, IHC, IP
• Clone: OTI9E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MIG2/Kindlin-2 Antibody (OTI9E4) Summary

• Immunogen: Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector
• Specificity: This antibody is specific for Homo sapiens fermitin family homolog 2 (Drosophila) (FERMT2), transcript variant 1.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FERMT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:500-1000
• Theoretical MW: 77.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MIG2/Kindlin-2 Antibody (OTI9E4)

• fermitin family homolog 2 (Drosophila)
• fermitin family homolog 2
• fermitin family member 2
• FERMT2
• KIND2
• KIND2DKFZp686G11125
• kindlin 2
• Kindlin-2
• MIG2
• MIG-2
• MIG2FLJ34213
• mitogen inducible gene 2 protein
• Mitogen-inducible gene 2 protein
• PH domain-containing family C member 1
• pleckstrin homology domain containing, family C (with FERM domain) member 1
• pleckstrin homology domain containing, family C member 1
• Pleckstrin homology domain-containing family C member 1
• PLEKHC1
• PLEKHC1FLJ44462
• UNC112
• UNC112B

Background

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MIG2/Kindlin-2 Antibody (NBP1-47745)(1)

Publication using NBP1-47745

• Liu G, Bao Y et al. IKK epsilon phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis. Theranostics 2020-02-28 [PMID: 32104508] (WB, Human)

Bioinformatics

• Gene Symbol: FERMT2
• Entrez:
  • 10979(Human)