MIG2/Kindlin-2 Antibody (OTI9E4)

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Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - GST-tagged wild-type MIG2/Kindlin-2 or MIG2/Kindlin-2 (S159A) were incubated without IKKE (-), or with purified wild-type IKKE (WT) or mutant IKKE (K38A) in ...read more
Immunocytochemistry/ Immunofluorescence: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of HepG2 cells using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human pancreas tissue using anti-Kindlin-2 mouse monoclonal antibody.
Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kindlin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts ...read more
Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Kindlin-2 monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human colon tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human Ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.
Immunoprecipitation: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
OTI9E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MIG2/Kindlin-2 Antibody (OTI9E4) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector
Specificity
This antibody is specific for Homo sapiens fermitin family homolog 2 (Drosophila) (FERMT2), transcript variant 1.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
FERMT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:500-1000
Theoretical MW
77.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-47745 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MIG2/Kindlin-2 Antibody (OTI9E4)

  • fermitin family homolog 2 (Drosophila)
  • fermitin family homolog 2
  • fermitin family member 2
  • FERMT2
  • KIND2
  • KIND2DKFZp686G11125
  • kindlin 2
  • Kindlin-2
  • MIG2
  • mig-2
  • MIG2FLJ34213
  • mitogen inducible gene 2 protein
  • Mitogen-inducible gene 2 protein
  • PH domain-containing family C member 1
  • pleckstrin homology domain containing, family C (with FERM domain) member 1
  • pleckstrin homology domain containing, family C member 1
  • Pleckstrin homology domain-containing family C member 1
  • PLEKHC1
  • PLEKHC1FLJ44462
  • UNC112
  • UNC112B

Background

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MIG2/Kindlin-2 Antibody (NBP1-47745)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FERMT2
Entrez