TSG101 Antibody (4A10)

Images

 
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Lane 1: MWM, Lane 2: TSG101 in RAW 264.7 Whole Cell Lysate, Lane 3: Exosome Lysate. Used primary antibody at 1:500 and Donkey Anti-Mouse at 0.5 ug/mL (this could be ...read more
Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - Paraffin-embedded Colon carcinoma, using antibody at 1:100 dilution.
Western Blot: TSG101 Antibody (4A10) [NB200-112] - A. 30 ug NIH-3T3 whole cell lysate/extract. B. 30 ug JC whole cell lysate/extract. C. 30 ug BCL-1 whole cell lysate/extract.
Western Blot: TSG101 Antibody (4A10) [NB200-112] - A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract.
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The signal was developed ...read more
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] ...read more
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated ...read more
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated ...read more
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE; antibody diluted at 1:1000.
ELISA: TSG101 Antibody (4A10) [NB200-112] - ELISA detection of TSG101 for capture at a concentration of 5 ug/mL and TSG101 for detection at a concentration of 1.5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, MkSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KO
Clone
4A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

TSG101 Antibody (4A10) Summary

Immunogen
Amino acids 167-374 of TSG101 protein expressed in E. coli.
Marker
Exosome Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TSG101
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry User should optimize the dilution
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated
Application Notes
Knockdown/Knockout usage was usage reported in scientific literature (PMID: 27226592). Suggested fixation for ICC/IF: 3-3.5% PFA with 0.05% saponin treatment.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
TSG101 Lysate (NBL1-17358)
Reviewed Applications
Read 2 Reviews rated 4
using
NB200-112 in the following applications:

Publications
Read Publications using
NB200-112 in the following applications:

  • 1 publication
  • 10 publications
  • IHC
    1 publication
  • IP
    3 publications
  • WB
    42 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for TSG101 Antibody (4A10)

  • ESCRT-I complex subunit TSG101
  • TSG10
  • tumor susceptibility gene 10
  • tumor susceptibility gene 101 protein
  • tumor susceptibility gene 101
  • tumor susceptibility protein
  • VPS23

Background

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phospho-protein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-112
Species: Hu, Mu, Rt, Ha, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KO

Publications for TSG101 Antibody (NB200-112)(67)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Hamster, Primate.

We have publications tested in 5 applications: ELISA, ICC/IF, IHC, IP, WB.


Filter By Application
ELISA
(1)
ICC/IF
(10)
IHC
(1)
IP
(3)
WB
(42)
All Applications
Filter By Species
Human
(46)
Mouse
(6)
Rat
(1)
Hamster
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 67. Show All 67 Publications.
Publications using NB200-112 Applications Species
Guix FX, Corbett GT, Cha DJ et al. Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles. Int J Mol Sci. Feb 27 2018 [PMID: 29495441] (ELISA, Human) ELISA Human
Boopathy GTK, Kulkarni M, Ho SY et al. Cavin-2 regulates the activity and stability of endothelial nitric oxide synthase (eNOS) in angiogenesis J. Biol. Chem. 2017 Sep 14 [PMID: 28912276] (Mouse) Mouse
Gschweitl M, Ulbricht A, Barnes CA et al. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes. Elife. 2016 Apr 22 [PMID: 27008177] (WB, Human) WB Human
Shabbir A, Coz A, Rodriguez L et al. Mesenchymal stem cell exosomes induce the proliferation and migration of normal and chronic wound fibroblasts, and enhance angiogenesis in vitro Stem Cells Dev. 2015 Apr 13 [PMID: 25867197]
Choy RW, Cheng Z, Schekman R et al. Amyloid precursor protein (APP) traffics from the cell surface via endosomes for amyloid B (AB) production in the trans-Golgi network. Proc Natl Acad Sci USA 2012 Jul [PMID: 22711829]
Toyofuku T, Nojima S, Ishikawa T, Takamatsu H, Tsujimura T, Uemura A, Matsuda J, Seki T, Kumanogoh A. Endosomal sorting by Semaphorin 4A in retinal pigment epithelium supports photoreceptor survival. Genes Dev;26(8):816-29. 2012 Apr 15. [PMID: 22465952] (WB, Human) WB Human
Nabhan JF, Hu R, Oh RS et al. Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein. Proc Natl Acad Sci USA 2012 Mar [PMID: 22315426] (WB, Human) WB Human
Rush JS, Quinalty LM, Engelman L et al. Endosomal accumulation of the activated epidermal growth factor receptor (EGFR) induces apoptosis. J Biol Chem 2012 Jan [PMID: 22102283]
Tampellini D, Rahman N, Lin MT et al. Impaired B-amyloid secretion in Alzheimer's disease pathogenesis. J Neurosci 2011 Oct [PMID: 22031884] (ICC/IF, Mouse) ICC/IF Mouse
Falguieres T, Castle D, Gruenberg J. Regulation of the MVB pathway by SCAMP3. Traffic 2012 Jan [PMID: 21951651]
Show All 67 Publications.

Reviews for TSG101 Antibody (NB200-112) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB200-112:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot TSG101 NB200-112
Enlarge
reviewed by:
Elizabeth Soberg
WB Mouse 06/05/2018
View

Summary

ApplicationWestern Blot
Sample TestedRAW 264.7 mouse monocyte/macrophage cell line
SpeciesMouse
Lot41449

Comments

CommentsUsed primary @ 1:500 and Donkey Anti-Mouse @ 0.5 ug/mL (this could be titrated down to reduce background). Used picogram sensitivity ECL reagents.
Western Blot TSG101 NB200-112
Enlarge
reviewed by:
Anonymous
WB Mouse 12/08/2017
View

Summary

ApplicationWestern Blot
Sample TestedPrimary mouse hepatocytes
SpeciesMouse
Lot41449

Comments

Commentsmouse hepatocytes were treated with 100 ng/ml LPS for 4 hours

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TSG101 Antibody (NB200-112) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-112AF405
Alexa Fluor 488 Labeled NB200-112AF488
Alexa Fluor 647 Labeled NB200-112AF647
Alexa Fluor 700 Labeled NB200-112AF700
Allophycocyanin Labeled NB200-112APC
DyLight 350 Labeled NB200-112UV
DyLight 405 Labeled NB200-112V
DyLight 488 Labeled NB200-112G
DyLight 680 Labeled NB200-112FR
DyLight 755 Labeled NB200-112IR
PE Labeled NB200-112PE
PerCP Labeled NB200-112PCP

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Bioinformatics Tool for TSG101 Antibody (NB200-112)

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Blogs on TSG101.

Tools for Isolation, Quantification and Analysis of Exosomes
Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Elizabeth Soberg
06/05/2018
Application: WB
Species: Mouse

Anonymous
12/08/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TSG101
Entrez
Uniprot