TSG101 Antibody (4A10)

Images

 
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Characterization of exosomes. Western blot analysis of exosomes revealed differential expression of TSG101 between HCT116-R exosomes and HCT116-P exosomes. Image ...read more
Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.TSG101 ...read more
Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] (NB200-112) detects TSG101 protein by flow cytometry analysis.Sample: THP-1 cell.Black: Unlabelled sample was used as a control.Red: TSG101 ...read more
Western Blot: TSG101 Antibody (4A10) [NB200-112] - A. 30 ug NIH-3T3 whole cell lysate/extract. B. 30 ug JC whole cell lysate/extract. C. 30 ug BCL-1 whole cell lysate/extract.
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated ...read more
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Lane 1: MWM, Lane 2: TSG101 in RAW 264.7 Whole Cell Lysate, Lane 3: Exosome Lysate. Used primary antibody at 1:500 and Donkey Anti-Mouse at 0.5 ug/mL (this could be ...read more
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated ...read more
Western Blot: TSG101 Antibody (4A10) [NB200-112] - K562 whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated ...read more
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse ...read more
Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.TSG101 ...read more
Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.TSG101 stained ...read more
Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - An intracellular stain was performed on HeLa cells with TSG101 Antibody (4A10) NB200-112F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - An intracellular stain was performed on Hek293 cells with TSG101 Antibody [4A10] NB200-112PE (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
ELISA: TSG101 Antibody (4A10) [NB200-112] - ELISA detection of TSG101 for capture at a concentration of 5 ug/mL and TSG101 for detection at a concentration of 1.5 ug/mL.
Knockdown Validated: TSG101 Antibody (4A10) [NB200-112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, Pm, PmSpecies Glossary
Applications WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD
Clone
4A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

TSG101 Antibody (4A10) Summary

Immunogen
Amino acids 167-374 of TSG101 protein expressed in E. coli.
Marker
Exosome Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TSG101
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Electron Microscopy 1:10
  • ELISA 1:100 - 1:2000
  • Flow Cytometry User should optimize the dilution
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
TSG101 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NB200-112 in the following applications:

Publications
Read Publications using
NB200-112 in the following applications:

  • 1 publication
  • 3 publications
  • 10 publications
  • IHC
    1 publication
  • IP
    3 publications
  • WB
    49 publications

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 18267010). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for TSG101 Antibody (4A10)

  • ESCRT-I complex subunit TSG101
  • TSG10
  • tumor susceptibility gene 10
  • tumor susceptibility gene 101 protein
  • tumor susceptibility gene 101
  • tumor susceptibility protein
  • VPS23

Background

Tumor susceptibility 101 protein (human TSG101 theoretical molecular weight 44kDa) is the mammalian homologue of the yeast protein Vps23 which plays a role in endosomal and multivesicular body trafficking. TSG101 forms part of the endosomal sorting complexes required for transport complex 1 (ESCRT-I), a cytosolic multiprotein complex consisting additionally of Vps28, Vps37 (A-D) and Mvb12 (A, B) or UBAP1 (1). TSG101 contains multiple domains including the amino terminal ubiquitin e2 variant (UEV) domain, a proline-rich (RRR) domain, a coiled coil (CC) domain, and a carboxy terminal alpha-helical/steadiness box (SB) domain (2). As part of the ESCRT-I complex, TSG101 interacts through its UEV domain with ubiquitinated membrane proteins and members of the ESCRT-0 complex. The UEV domain plays a critical role for various TSG101 functions such as protein sorting into multivesicular bodies and late endosomes, and in the process of viral budding. For example, TSG101 interacts with the intermediate-conductance, Ca2+ -activated K+ channel (KCa3.1), facilitating its targeting to the lysosome for degradation (3). Additionally, TSG101 has been implicated in the turnover of connexins such as connexins 43 and 45 (4). TSG101 plays a role in other cellular functions including transcriptional regulation, cytokinesis and cell growth (2).

Upon its initial discovery, TSG101 was recognized as a tumor suppressor protein due to the identification of deletions within the TSG101 gene in human breast carcinomas. However, re-examination of the initial findings argued against this function and supported that TSG101 promotes tumorigenesis (2). In agreement with this role, TSG101 expression is upregulated in several types of cancer including breast, ovarian, and colorectal carcinoma.

References

1. Schmidt, O., & Teis, D. (2012). The ESCRT machinery. Current Biology. https://doi.org/10.1016/j.cub.2012.01.028

2. Jiang, Y., Ou, Y., & Cheng, X. (2013). Role of TSG101 in cancer. Frontiers in Bioscience. https://doi.org/10.2741/4099

3. Balut, C. M., Gao, Y., Murray, S. A., Thibodeau, P. H., & Devor, D. C. (2010). ESCRT-dependent targeting of plasma membrane localized KCa3.1 to the lysosomes. American Journal of Physiology - Cell Physiology. https://doi.org/10.1152/ajpcell.00120.2010

4. Su, V., & Lau, A. F. (2014). Connexins: Mechanisms regulating protein levels and intercellular communication. FEBS Letters. https://doi.org/10.1016/j.febslet.2014.01.013

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-112
Species: Hu, Mu, Rt, Ha, Pm, Pm
Applications: WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD

Publications for TSG101 Antibody (NB200-112)(77)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Hamster, Primate.

We have publications tested in 6 applications: ELISA, Flow, ICC/IF, IHC, IP, WB.


Filter By Application
ELISA
(1)
Flow
(3)
ICC/IF
(10)
IHC
(1)
IP
(3)
WB
(49)
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Filter By Species
Human
(54)
Mouse
(8)
Rat
(1)
Hamster
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 77. Show All 77 Publications.
Publications using NB200-112 Applications Species
Deng Z, Wang Y, Hu L et al. A facile, rapid, high-throughput extracellular vesicles analytical platform for cancer detection Analytica Chimica Acta Nov 1 2020 [PMID: 33161974] (WB, Human) WB Human
Zhou X, Brown BA, Siegel AP et al. Exosome-Mediated Crosstalk between Keratinocytes and Macrophages in Cutaneous Wound Healing ACS Nano Sep 15 2020 [PMID: 32931251] (Flow) Flow
Kwon MH, Song KM, Limanjaya A, et al. Embryonic stem cell-derived extracellular vesicle-mimetic nanovesicles rescue erectile function by enhancing penile neurovascular regeneration in the streptozotocin-induced diabetic mouse Sci Rep Dec 27 2019 [PMID: 31882614] (WB, Mouse) WB Mouse
Uygur B, Melikov K, Arakelyan A et al. Syncytin 1 dependent horizontal transfer of marker genes from retrovirally transduced cells Sci Rep Nov 27 2019 [PMID: 31776415] (WB, Human, Mouse) WB Human, Mouse
Hon KW, Ab-Mutalib NS, Abdullah NMA et al. Extracellular Vesicle-derived circular RNAs confers chemoresistance in Colorectal cancer Sci Rep Nov 11 2019 [PMID: 31712601] (WB, Human) WB Human
Murdica, V;Giacomini, E;Alteri, A;Bartolacci, A;Cermisoni, GC;Zarovni, N;Papaleo, E;Montorsi, F;Salonia, A;Vigano, P;Vago, R; Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation Fertil. Steril. May 1 2019 [PMID: 31029245] (WB, Human) WB Human
Christodoulou K, Minaidou A, Nicolaou P. Deregulation of LRSAM1 expression impairs the levels of TSG101, UBE2N, VPS28, MDM2 and EGFR PLoS One. [PMID: 30726272] (WB, Human) WB Human
Hough kP, Trevor JL, Strenkowski JG et al. Exosomal transfer of mitochondria from airway myeloid-derived regulatory cells to T cells. Redox Biol. Jun 25 2018 [PMID: 29986209] (Flow, Human) Flow Human
Hough kP, Wilson LS, Trevor JL et al. Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics. Sci Rep. Jul 9 2018 [PMID: 29985427] (Flow, Human) Flow Human
Guix FX, Corbett GT, Cha DJ et al. Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles. Int J Mol Sci. Feb 27 2018 [PMID: 29495441] (ELISA, Human) ELISA Human
Show All 77 Publications.

Reviews for TSG101 Antibody (NB200-112) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB200-112:
Filter by Applications
WB
(2)
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Filter by Species
Mouse
(2)
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Images Ratings Applications Species Date Details
Western Blot TSG101 NB200-112
Enlarge
4
reviewed by:
Elizabeth Soberg
WB Mouse 06/05/2018
View

Summary

ApplicationWestern Blot
Sample TestedRAW 264.7 mouse monocyte/macrophage cell line
SpeciesMouse
Lot41449

Comments

CommentsUsed primary @ 1:500 and Donkey Anti-Mouse @ 0.5 ug/mL (this could be titrated down to reduce background). Used picogram sensitivity ECL reagents.
Western Blot TSG101 NB200-112
Enlarge
4
reviewed by:
Anonymous
WB Mouse 12/08/2017
View

Summary

ApplicationWestern Blot
Sample TestedPrimary mouse hepatocytes
SpeciesMouse
Lot41449

Comments

Commentsmouse hepatocytes were treated with 100 ng/ml LPS for 4 hours

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TSG101 Antibody (NB200-112) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
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Elizabeth Soberg
06/05/2018
Application: WB
Species: Mouse

Anonymous
12/08/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TSG101
Entrez
Uniprot