VPS4B Antibody

Images

 
Western Blot: VPS4B Antibody [NBP2-20881] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:3000.
Immunocytochemistry/ Immunofluorescence: VPS4B Antibody [NBP2-20881] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B protein stained by VPS4B antibody [N3C3] diluted at 1:500. Red: alpha ...read more
Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-20881] - Paraffin-embedded colon carcinoma. VPS4B antibody [N3C3] dilution: 1:500.
Immunocytochemistry/ Immunofluorescence: VPS4B Antibody [NBP2-20881] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B protein stained by VPS4B antibody [N3C3] diluted at 1:500. Blue: Hoechst ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.48 mg/ml

Order Details

VPS4B Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human VPS4B. The exact sequence is proprietary.
Localization
Prevacuolar compartment membrane; Peripheral membrane protein; Late endosome membrane; Peripheral membrane protein (Probable)
Predicted Species
Mouse (96%), Rat (96%), Rhesus Macaque (99%), Bovine (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS4B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (88%), Xenopus laevis (88%), Chicken (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.48 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS4B Antibody

  • Cell migration-inducing gene 1 protein
  • Protein SKD1
  • SKD1B
  • SKD1cell migration-inducing 1
  • Suppressor of K(+) transport growth defect 1
  • suppressor of K+ transport defect 1
  • vacuolar protein sorting 4 homolog B (S. cerevisiae)
  • vacuolar protein sorting 4B (yeast)
  • vacuolar protein sorting 4B
  • vacuolar protein sorting-associated protein 4B
  • VPS42
  • VPS4-2

Background

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol VPS4B
Uniprot