VPS4B Antibody

Western Blot: VPS4B Antibody [NBP1-19119] - Whole cell lysate from HeLa and 293T cells. VPS4B was also immunoprecipitated by rabbit anti-VPS4B antibody NBP1-19118.

• Reactivity: Hu, Pm-Or
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

VPS4B Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human vacuolar protein sorting 4 homolog B using the numbering given in entry NP_004860.2 (GeneID 9525).
• Predicted Species: Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS4B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VPS4B Antibody

• Cell migration-inducing gene 1 protein
• Protein SKD1
• SKD1B
• SKD1cell migration-inducing 1
• Suppressor of K(+) transport growth defect 1
• suppressor of K+ transport defect 1
• vacuolar protein sorting 4 homolog B (S. cerevisiae)
• vacuolar protein sorting 4B (yeast)
• vacuolar protein sorting 4B
• vacuolar protein sorting-associated protein 4B
• VPS42
• VPS4-2

Background

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VPS4B Antibody (NBP1-19119)(2)

Publications using NBP1-19119

• Du X, Kazim AS, Dawes IW et al. The AAA ATPase VPS4/SKD1 regulates endosomal cholesterol trafficking independently of ESCRT-III.. Traffic. 2013-01-01 [PMID: 23009658] (WB, Human)
• Ariumi Y, Kuroki M, Maki M et al. The ESCRT System Is Required for Hepatitis C Virus Production. PLoS One. 2011-01-11 [PMID: 21264300]

Bioinformatics

• Gene Symbol: VPS4B
• Entrez:
  • 9525(Human)
• Uniprot:
  • O75351(Human)