Vinculin Antibody (VIN-11-5)

Images

 
Western Blot: Vinculin Antibody (VIN-11-5) [NB120-11193] - Chicken gizzard cell extract was separated on SDS-PAGE and probed with NB120-11193. The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a ...read more
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (VIN-11-5) [NB120-11193] - Mouse adult fibroblast stained with Monoclonal Anti-Vinculin, clone VIN-11-5 at a 1:40 dilution (aqua). Actin (purple) and nuclear ...read more
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (VIN-11-5) [NB120-11193] - Double labeling of an isolated smooth muscle cell (chicken gizzard) with Monoclonal Anti-Vinculin

Product Details

Summary
Reactivity Hu, Mu, Bv, ChSpecies Glossary
Applications WB, ICC/IF, MiAr
Clone
VIN-11-5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Vinculin Antibody (VIN-11-5) Summary

Immunogen
smooth muscle vinculin from chicken gizzard.
Localization
Cytoplasmic
Marker
Focal Adhesion Marker
Specificity
The reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VCL
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Microarray
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Vinculin Lysate (NBL1-17706)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Vinculin Antibody (VIN-11-5)

  • CMD1W
  • CMH15
  • metavinculin
  • MVCL
  • VCL
  • Vinculin

Background

Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell extracellular matrix adherens type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. The sequences of chicken, nematode and human vinculin have been determined. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha actinin and possibly with itself. Since alpha actinin binds to and cross links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Vinculin Antibody (NB120-11193). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any 100 coda or bigger controls?
    • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VCL
Entrez
Uniprot