Vinculin Antibody (728526) [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vinculin Monoclonal ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Vinculin at approximately ...read more
Vinculin was detected in immersion fixed paraffin-embedded sections of human uterus using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) at 15 µg/mL overnight at 4 °C. Before incubation with the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clone
728526
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Vinculin Antibody (728526) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Vinculin
Lys1020-Gln1134
Accession # P18206
Specificity
Detects human Vinculin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
VCL
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Simple Western 20 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4.3
using
MAB6896 in the following applications:

Publications
Read Publications using
MAB6896 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vinculin Antibody (728526) [Unconjugated]

  • CMD1W
  • CMH15
  • metavinculin
  • MVCL
  • VCL
  • Vinculin

Background

VCL (vinculin) is a 117 kDa actin binding cytoskeletal phosphoprotein found in adherens-type cell-cell and cell-matrix junctions. The 1066 amino acid (aa) human vinculin contains an N-terminal globular head (aa 2-835) with a talin interaction domain (aa 168-208) and three tandem repeats (aa 259-589), a pro-rich linker region (aa 826-878), and a C-terminal tail that facilitates phospholipid membrane insertion (aa 1003-1134). An 1134 aa form termed metavinculin contains 68 aa inserted after aa 915 and mainly occurs in cardiac, skeletal and smooth muscle. Human, mouse and rat vinculin share 100% aa sequence identity within the region used as an immunogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Vinculin Antibody (MAB6896)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Western Blot, Western Blot Control.


Filter By Application
Western Blot
(4)
Western Blot Control
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
All Species
Showing Publications 1 - 5 of 5.
Publications using MAB6896 Applications Species
CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells Cell Rep, 2019;28(13):3329-3337.e5. 2019 [PMID: 31553904] (Western Blot, Human) Western Blot Human
A Schramm, P Mueller-Th, T Littmann, M Harloff, T Ozawa, J Schlossman Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies Int J Mol Sci, 2018;19(4):. 2018 [PMID: 29649180] (Western Blot, Mouse) Western Blot Mouse
A Baradaran-, J Niesser, AD Balgi, K Choi, C Zimmerman, AP South, HJ Anderson, NC Strynadka, MB Bally, M Roberge Gentamicin B1 is a minor gentamicin component with major nonsense mutation suppression activity Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28289221] (Western Blot Control, Mouse) Western Blot Control Mouse
Alireza Baradaran-, Aruna D Balgi, Carla Zimmerman, Kunho Choi, Fahimeh S Shidmooss, Jason S Tan, Célia Bergeaud, Alexandra Krause, Stéphane Flibotte, Yoko Shimizu, Hilary J Anderson, Vincent Mouly, Eric Jan, Tom Pfeifer, James B Jaquith, Michel Roberge Novel small molecules potentiate premature termination codon readthrough by aminoglycosides. Nucleic Acids Research, 2016;0(0):1362-4962. 2016 [PMID: 27407112] (Western Blot, Human) Western Blot Human
Naresh C Bal Increased reliance on muscle based thermogenesis upon acute minimization of brown adipose tissue function J Biol Chem, 2016;0(0):. 2016 [PMID: 27298322] (Western Blot, Mouse) Western Blot Mouse

Reviews for Vinculin Antibody (MAB6896) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using MAB6896:
Filter by Applications
Simple Western
(2)
WB
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western Vinculin MAB6896
Enlarge
5
reviewed by:
Krishna Yalla
Simple Western Human 08/27/2020
View

Summary

ApplicationSimple Western
Sample TestedHCT116 cell line lysate
SpeciesHuman
LotCFT00117091

Comments

CommentsAb titration on HCT116 cell lysate
Western Blot Vinculin MAB6896
Enlarge
4
reviewed by:
Anonymous
WB Human 10/16/2015
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
LotCFTO0112081

Blocking

Blocking DetailsBlocker=BSA, Buffer= 1XTris Buffer Saline+0.1 % Tween 20, 2hrs at RT

Primary Anitbody

Dilution Ratio1:500 over night at 4 degree Celsius

Secondary Antibody

Secondary Descriptionanti-Mouse IgG, HRP
Secondary Manufacturer Cat#ab20043
Secondary Concentration1:20,000

Details

Detection Notes30 seconds exposure

Comments

CommentsYeah! it works
Simple Western Vinculin MAB6896
Enlarge
4
reviewed by:
Asimina Kerimi
Simple Western Mouse 09/23/2015
View

Summary

ApplicationSimple Western
Sample TestedCaCo2 whole cell lysate
SpeciesMouse
LotCFTO011508A

Blocking

Blocking Details30 min incubation on Wes platform

Secondary Antibody

Secondary DescriptionPS provided mouse HRP
Secondary Manufacturer Cat#n/a

Details

Detection NotesWES Protein Simple, Multi-image analysis used

Comments

CommentsCell lysate was collected in Bicine-Chaps Buffer

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Vinculin Antibody (MAB6896). (Showing 1 - 1 of 1 FAQs).

  1. Do you have any 100 coda or bigger controls?
    • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for Vinculin Antibody (MAB6896)

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Pathways for Vinculin Antibody (MAB6896)

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Blogs on Vinculin

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Krishna Yalla
08/27/2020
Application: Simple Western
Species: Human

Anonymous
10/16/2015
Application: WB
Species: Human

Asimina Kerimi
09/23/2015
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol VCL
Uniprot