| Reactivity | Hu, Mu, Rt, Po, Ca, Ch, Gt, Ha, Pm, Xp, ZeSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P |
| Clone | RV202 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Janelia Fluor 549 |
| Immunogen | Clone RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens. |
| Specificity | RV202 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | VIM |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![Vimentin Antibody (RV202) [Janelia Fluor® 549]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Vimentin Antibody (RV202) [Janelia Fluor® 549]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP1-97672AF350 | |
| Alexa Fluor 405 | NBP1-97672AF405 | |
| Alexa Fluor 488 | NBP1-97672AF488 | |
| Alexa Fluor 532 | NBP1-97672AF532 | |
| Alexa Fluor 594 | NBP1-97672AF594 | |
| Alexa Fluor 647 | NBP1-97672AF647 | |
| Alexa Fluor 700 | NBP1-97672AF700 | |
| Alexa Fluor 750 | NBP1-97672AF750 | |
| Allophycocyanin | NBP1-97672APC | |
| Biotin | NBP1-97672B | |
| DyLight 350 | NBP1-97672UV | |
| DyLight 405 | NBP1-97672V | |
| DyLight 488 | NBP1-97672G | |
| DyLight 550 | NBP1-97672R | |
| DyLight 594 | NBP1-97672DL594 | |
| DyLight 650 | NBP1-97672C | |
| DyLight 680 | NBP1-97672FR | |
| DyLight 755 | NBP1-97672IR | |
| FITC | NBP1-97672F | |
| HRP | NBP1-97672H | |
| Janelia Fluor 549 | NBP1-97672JF549 | |
| Janelia Fluor 646 | NBP1-97672JF646 | |
| PE | NBP1-97672PE | |
| PerCP | NBP1-97672PCP |
Diseases for Vimentin Antibody (NBP1-97672JF549)Discover more about diseases related to Vimentin Antibody (NBP1-97672JF549).
| Pathways for Vimentin Antibody (NBP1-97672JF549)View related products by pathway.
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Antibody treatment can generate microglia-like cells from bone marrow By Jennifer Sokolowski, MD, PhD.Microglia play important roles in the brain in both homeostatic and pathological conditions, acting to clear debris and dying cells. There is evidence to suggest that microglial dys... Read full blog post. |
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Stemness is responsible for onset and metastasis of colorectal cancer By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C... Read full blog post. |
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The use of Beta Actin (AC-15) as a loading control across multiple species Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs. Actin has six highly conserved isoforms, however beta and gamma actin are the two... Read full blog post. |
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The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation. Just like any other model organism, it is important to have an effective loading control to va... Read full blog post. |
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Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t... Read full blog post. |
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Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post. |
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CD63: is it pro-metastatic or anti-metastatic? CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,... Read full blog post. |
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Vimentin: Regulating EMT and Cancer Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea... Read full blog post. |
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Vimentin in Wound Healing Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p... Read full blog post. |
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Vimentin as a Marker for Epithelial-to-Mesenchymal Transition Epithelial-to-mesenchymal transition (EMT) is a critical event in the induction of cell motility and increased survival both under physiological situations like wound healing or development, as well as in malignant cells undergoing invasion and metast... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | VIM |
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.](http://images.novusbio.com/images2/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).](http://images.novusbio.com/images/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Xenografted mouse brain section: astocyte and human nuclei. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MUC1 [SM3] at 5 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/MUC-1-Antibody-(SM3)-Immunocytochemistry-Immunofluorescence-NB120-22711-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The xenograft section depicted a very specific and intense signal in the periphery of the cancer cells. The necrotic cells also developed a strong immune-positivity while the tumor stroma as well as the nuclei of cells were negative for immunostaining.](http://images.novusbio.com/images2/MUC-1-Antibody-(SM3)-Immunohistochemistry-Paraffin-NB120-22711-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MUC-1 antibody was tested in MCF7 cells at 10 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.](http://images.novusbio.com/images2/MUC-1-Antibody-(SM3)-Immunocytochemistry-Immunofluorescence-NB120-22711-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/CD34 Antibody (MEC 14.7)-Immunocytochemistry-Immunofluorescence-NB600-1071-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Immunohistochemistry-Paraffin-NB600-1071-img0014.jpg)
![Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Flow-Cytometry-NB600-1071-img0015.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.](http://images.novusbio.com/images2/Enolase-2-Neuron-specific-Enolase-Antibody-Western-Blot-NB110-58870-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of mixed cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:500 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. the NSE antibody labels protein expressed in neuronal cells, while the GFAP antibody stains intermediate filaments in astrocytic and certain other glial cells.](http://images.novusbio.com/images2/Enolase-2-Neuron-specific-Enolase-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58870-img0006.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.](http://images.novusbio.com/images2/Enolase-2-Neuron-specific-Enolase-Antibody-Western-Blot-NB110-58870-img0005.jpg)
![Western Blot: ETFA Antibody [NBP2-16382] - Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/ETFA-Antibody-Western-Blot-NBP2-16382-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: ETFA Antibody [NBP2-16382] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ETFA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/images2/ETFA Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16382-img0005.jpg)
![Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody dilution: 1:500.](http://images.novusbio.com/images2/ETFA-Antibody-Immunohistochemistry-Paraffin-NBP2-16382-img0010.jpg)
![Western Blot: S100B Antibody (15F9NB) [NBP2-45267] - Total protein from human brain (1) and mouse brain (2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/S100B-Antibody-(15F9NB)-Western-Blot-NBP2-45267-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/S100B-Antibody-(15F9NB)-Immunocytochemistry-Immunofluorescence-NBP2-45267-img0011.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in human prostate tissue.](http://images.novusbio.com/images2/S100B-Antibody-(15F9NB)-Immunohistochemistry-Paraffin-NBP2-45267-img0014.jpg)
![Western Blot: S100A1 Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.](http://images.novusbio.com/images2/S100A1-Antibody-(4C4.9)-Western-Blot-NBP2-29403-img0001.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100 Ab (4C4.9).](http://images.novusbio.com/images2/S100A1-Antibody-(4C4.9)-Immunohistochemistry-Paraffin-NBP2-29403-img0003.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100 4C4.9 antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining.](http://images.novusbio.com/images2/S100A1-Antibody-(4C4.9)-Immunohistochemistry-Paraffin-NBP2-29403-img0002.jpg)
![Western Blot: Fibronectin Antibody [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (http://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Fibronectin-Antibody-Western-Blot-NBP1-91258-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0012.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunohistochemistry-Frozen-NBP1-91258-img0019.jpg)
![Western Blot: SLC22A3 Antibody [NBP1-69368] - This Anti-SLC22A3 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 0.5ug/ml.](http://images.novusbio.com/images2/SLC22A3-Antibody-Western-Blot-NBP1-69368-img0001.jpg)
![Immunohistochemistry: SLC22A3 Antibody [NBP1-69368] - Human kidney](http://images.novusbio.com/images2/SLC22A3-Antibody-Immunohistochemistry-NBP1-69368-img0002.jpg)
![Western Blot: ITK Antibody (5G6) [NBP2-37578] - Analysis using ITK mouse mAb against Jurkat cell lysate.](http://images.novusbio.com/images2/ITK-Antibody-(5G6)-Western-Blot-NBP2-37578-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ITK Antibody (5G6) [NBP2-37578] - Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/images2/ITK-Antibody-(5G6)-Immunocytochemistry-Immunofluorescence-NBP2-37578-img0004.jpg)
![Flow Cytometry: ITK Antibody (5G6) [NBP2-37578] - Analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).](http://images.novusbio.com/images2/ITK-Antibody-(5G6)-Flow-Cytometry-NBP2-37578-img0005.jpg)
![Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.](http://images.novusbio.com/images2/Synaptophysin-Antibody-Western-Blot-NB300-653-img0001.jpg)
![Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653] - Feline retina. Synaptophysin (green), DAPI (blue). The image was capture by epifluorescent microscopy. Image from verified customer review.](http://images.novusbio.com/images2/Synaptophysin-Antibody-Immunohistochemistry-Frozen-NB300-653-img0006.jpg)
![Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.](http://images.novusbio.com/images2/Synaptophysin-Antibody-Immunohistochemistry-Paraffin-NB300-653-img0002.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)