Vimentin Antibody (RV202) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Ch, Gt, Ha, Pm, Xp, ZeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
RV202
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Vimentin Antibody (RV202) [Janelia Fluor® 549] Summary

Immunogen
Clone RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
Specificity
RV202 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VIM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Vimentin Antibody (RV202) [Janelia Fluor® 549]

  • epididymis secretory sperm binding protein
  • FLJ36605
  • VIM
  • Vimentin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-97672JF549
Species: Hu, Mu, Rt, Po, Ca, Ch, Gt, Ha, Pm, Xp, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Vimentin Antibody (NBP1-97672JF549) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-97672AF350
Alexa Fluor 405 NBP1-97672AF405
Alexa Fluor 488 NBP1-97672AF488
Alexa Fluor 532 NBP1-97672AF532
Alexa Fluor 594 NBP1-97672AF594
Alexa Fluor 647 NBP1-97672AF647
Alexa Fluor 700 NBP1-97672AF700
Alexa Fluor 750 NBP1-97672AF750
Allophycocyanin NBP1-97672APC
Biotin NBP1-97672B
DyLight 350 NBP1-97672UV
DyLight 405 NBP1-97672V
DyLight 488 NBP1-97672G
DyLight 550 NBP1-97672R
DyLight 594 NBP1-97672DL594
DyLight 650 NBP1-97672C
DyLight 680 NBP1-97672FR
DyLight 755 NBP1-97672IR
FITC NBP1-97672F
HRP NBP1-97672H
Janelia Fluor 549 NBP1-97672JF549
Janelia Fluor 646 NBP1-97672JF646
PE NBP1-97672PE
PerCP NBP1-97672PCP

Additional Vimentin Products

Bioinformatics Tool for Vimentin Antibody (NBP1-97672JF549)

Discover related pathways, diseases and genes to Vimentin Antibody (NBP1-97672JF549). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Vimentin Antibody (NBP1-97672JF549)

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Blogs on Vimentin. Showing 1-10 of 12 blog posts - Show all blog posts.

Antibody treatment can generate microglia-like cells from bone marrow
By Jennifer Sokolowski, MD, PhD.Microglia play important roles in the brain in both homeostatic and pathological conditions, acting to clear debris and dying cells. There is evidence to suggest that microglial dys...  Read full blog post.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Vimentin in Wound Healing
Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p...  Read full blog post.

Vimentin as a Marker for Epithelial-to-Mesenchymal Transition
Epithelial-to-mesenchymal transition (EMT) is a critical event in the induction of cell motility and increased survival both under physiological situations like wound healing or development, as well as in malignant cells undergoing invasion and metast...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol VIM