Vimentin Antibody (280618) [Unconjugated]


Vimentin was detected in immersion fixed A549 human lung carcinoma cell line using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were more
Western blot shows lysates of MEF mouse embryonic feeder cells, NIH‑3T3 mouse embryonic fibroblast cell line, Rat‑2 rat embryonic fibroblast cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane more
A172 human glioblastoma cell line was stained with Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by more
Vimentin was detected in immersion fixed paraffin-embedded sections of human tonsil using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 0.5 µg/mL for 1 hour at room temperature more
Vimentin was detected in immersion fixed rat cortical stem cells using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the more
Vimentin was detected in immersion fixed mouse cortical stem cells using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using more
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Vimentin at approximately 58 kDa (as indicated) using 10 µg/mL of more
Vimentin was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, Flow, ICC/IF, KO

Order Details

Vimentin Antibody (280618) [Unconjugated] Summary

E. coli-derived recombinant human Vimentin
Accession # P08670
Vimentin antibodies are ideal for immunocytochemistry colocalization studies in intermediate filaments. Detects human, mouse and rat Vimentin in Western blots.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 0.5-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 1-2 ug/mL
Reviewed Applications
Read 1 Review rated 5
MAB2105 in the following applications:

Read Publications using
MAB2105 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vimentin Antibody (280618) [Unconjugated]

  • epididymis secretory sperm binding protein
  • FLJ36605
  • VIM
  • Vimentin


Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine, and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9-11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).

  1. Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.
  2. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
  3. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.
  4. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
  5. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
  6. Li, Q-F. et al. (2006) J. Biol. Chem. 281:34716.
  7. Esue, O. et al. (2006) J. Biol. Chem. 281:30393.
  8. Styers, M.L. et al. (2005) Traffic 6:359.
  9. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
  10. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
  11. Ivaska, J. et al. (2005) EMBO J. 24:3834.
  12. Byun, Y. et al. (2001) Cell Death Differ. 8:443.
  13. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
  14. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
  15. Garg, A. et al. (2006) J. Immunol. 177:6192.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Vimentin Antibody (MAB2105)(17)

We have publications tested in 4 confirmed species: Human, Mouse, Rabbit, Xenograft.

We have publications tested in 6 applications: Flow Cytometry, ICC, IF, IHC, IHC-P, Western Blot.

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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using MAB2105 Applications Species
C Rodriguez-, N Kale, MJ Carlini, N Shrivastav, AA Rodrigues, B Khalil, JJ Bravo-Cord, M Alexander, J Ji, Y Hong, F Behbod, MS Sosa NR2F1 is a barrier to dissemination of early stage breast cancer cells Cancer Research, 2022;0(0):. 2022 [PMID: 35471456] (IF, Mouse) IF Mouse
MM Capeling, S Huang, CJ Childs, JH Wu, YH Tsai, A Wu, N Garg, EM Holloway, N Sundaram, C Bouffi, M Helmrath, JR Spence Suspension culture promotes serosal mesothelial development in human intestinal organoids Cell Reports, 2022;38(7):110379. 2022 [PMID: 35172130] (IHC, Human) IHC Human
de Rus Jacquet A, Tancredi J, Lemire A, DeSantis M, Li W, O'Shea E The LRRK2 G2019S mutation alters astrocyte-to-neuron communication via extracellular vesicles and induces neuron atrophy in a human iPSC-derived model of Parkinson's disease. Elife, 0;10(0):. 0 [PMID: 34590578] (ICC, Human) ICC Human
M Cui, A Atmanli, MG Morales, W Tan, K Chen, X Xiao, L Xu, N Liu, R Bassel-Dub, EN Olson Nrf1 promotes heart regeneration and repair by regulating proteostasis and redox balance Nature Communications, 2021;12(1):5270. 2021 [PMID: 34489413] (IHC, Mouse) IHC Mouse
GT Park, JW Yoon, SB Yoo, YC Song, P Song, HK Kim, J Han, SJ Bae, KT Ha, NP Mishchenko, SA Fedoreyev, VA Stonik, MB Kim, JH Kim Echinochrome A Treatment Alleviates Fibrosis and Inflammation in Bleomycin-Induced Scleroderma Marine Drugs, 2021;19(5):. 2021 [PMID: 33922418] (IHC, Mouse) IHC Mouse
LA Tashireva, OE Savelieva, ES Grigoryeva, YV Nikitin, EV Denisov, SV Vtorushin, MV Zavyalova, NV Cherdyntse, VM Perelmuter Heterogeneous Manifestations of Epithelial-Mesenchymal Plasticity of Circulating Tumor Cells in Breast Cancer Patients International Journal of Molecular Sciences, 2021;22(5):. 2021 [PMID: 33801519] (Flow Cytometry, Human) Flow Cytometry Human
D Yamakawa, D Katoh, K Kasahara, T Shiromizu, M Matsuyama, C Matsuda, Y Maeno, M Watanabe, Y Nishimura, M Inagaki Primary cilia-dependent lipid raft/caveolin dynamics regulate adipogenesis Cell Reports, 2021;34(10):108817. 2021 [PMID: 33691104] (ICC, Mouse) ICC Mouse
SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity Nature Communications, 2021;12(1):262. 2021 [PMID: 33431859] (IHC, Human) IHC Human
Z Wang, M Cui, AM Shah, W Tan, N Liu, R Bassel-Dub, EN Olson Cell-Type-Specific Gene Regulatory Networks Underlying Murine Neonatal Heart Regeneration at Single-Cell Resolution Cell Reports, 2020;33(10):108472. 2020 [PMID: 33296652] (ICC, Human) ICC Human
P Saikia, JS Crabb, LL Dibbin, MJ Juszczak, B Willard, GF Jang, TM Shiju, JW Crabb, SE Wilson Quantitative proteomic comparison of myofibroblasts derived from bone marrow and cornea Sci Rep, 2020;10(1):16717. 2020 [PMID: 33028893] (ICC, Rabbit) ICC Rabbit
Show All 17 Publications.

Review for Vimentin Antibody (MAB2105) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB2105:
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Vimentin MAB2105
reviewed by:
Verified Customer
IHC-Fr Human 02/22/2019


Sample TestedCultured Human Keratinocytes

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Blogs on Vimentin. Showing 1-10 of 14 blog posts - Show all blog posts.

Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.

  Read full blog post.

Antibody treatment can generate microglia-like cells from bone marrow
By Jennifer Sokolowski, MD, PhD.Microglia play important roles in the brain in both homeostatic and pathological conditions, acting to clear debris and dying cells. There is evidence to suggest that microglial dys...  Read full blog post.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.
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Verified Customer
Application: IHC-Fr
Species: Human


Gene Symbol VIM