VGLUT1 Antibody

Images

 
Western Blot: VGLUT1 Antibody [NBP3-13165] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: VGLUT1 Antibody [NBP3-13165] - VGLUT1 antibody detects VGLUT1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% ...read more
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165] - Rat brain. VGLUT1 stained by VGLUT1 antibody (NBP3-13165) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: VGLUT1 Antibody [NBP3-13165] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGLUT1 antibody (NBP3-13165) diluted at 1:5000. The HRP-conjugated ...read more
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165] - Rat brain. VGLUT1 antibody (NBP3-13165) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Frozen: VGLUT1 Antibody [NBP3-13165] - VGLUT1 antibody detects VGLUT1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: VGLUT1 protein ...read more
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165] - Mouse cerebellum. VGLUT1 stained by VGLUT1 antibody (NBP3-13165) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165] - Mouse brain. VGLUT1 stained by VGLUT1 antibody (NBP3-13165) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165] - Rat brain. VGLUT1 stained by VGLUT1 antibody (NBP3-13165) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165] - Mouse brain. VGLUT1 stained by VGLUT1 antibody (NBP3-13165) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165] - Mouse brain. VGLUT1 stained by VGLUT1 antibody (NBP3-13165) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-13165] - Mouse brain. VGLUT1 stained by VGLUT1 antibody (NBP3-13165) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

VGLUT1 Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of mouse VGLUT1. The exact sequence is proprietary.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC17A7
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS pH7, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for VGLUT1 Antibody

  • BNPI
  • BNPIbrain-specific Na-dependent inorganic phosphate cotransporter
  • Brain-specific Na(+)-dependent inorganic phosphate cotransporter
  • member 7
  • SLC17A7
  • solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter)
  • Solute carrier family 17 member 7
  • VGLUT1
  • VGLUT1vesicular glutamate transporter 1

Background

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC17A7