VAChT/SLC18A3 Antibody (S6-38) - BSA Free

Western Blot: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Western Blot analysis of Rat brain membrane lysate showing detection of VAChT/SLC18A3 protein using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:1000.

Immunocytochemistry/Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VAChT/SLC18A3 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38. Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S6-38
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

VAChT/SLC18A3 Antibody (S6-38) - BSA Free Summary

• Description: Novus Biologicals Mouse VAChT/SLC18A3 Antibody (S6-38) - BSA Free (NBP2-59378) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-VAChT/SLC18A3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide amino acids 521-532 of human VAChT
• Localization: Membrane
• Specificity: Detects 56kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC18A3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for VAChT/SLC18A3 Antibody (S6-38) - BSA Free

• MGC12716
• SLC18A3
• solute carrier family 18 (vesicular acetylcholine), member 3
• Solute carrier family 18 member 3
• solute carrier family 18, member 3
• VAChT
• VACHTvesicular acetylcholine transporter

Background

Vesicular Acetylcholine Transporter (VAChT), (approx. 70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer's disease and other nervous system disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAChT/SLC18A3 Antibody (NBP2-59378)(2)

Publications using NBP2-59378

• Farid H, Gelford WB, Goss LL et al. Fast Blue and Cholera Toxin-B Survival Guide for Alpha-Motoneurons Labeling: Less Is Better in Young B6SJL Mice, but More Is Better in Aged C57Bl/J Mice Bioengineering (Basel, Switzerland) 2023-01-20 [PMID: 36829635] (IHC, Mouse)
• Suzuki K, Ohi Y, Sato T et al. Reduction of glutamatergic activity through cholinergic dysfunction in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice Research Square 2022-09-29 [PMID: 36357544]

Bioinformatics

• Gene Symbol: SLC18A3