VG5Q Antibody - Azide and BSA Free

Western Blot: VG5Q Antibody [NB100-454] - Human VG5Q detected in HT1080 non-transfected cell lysate (NTC) and HT1080 transfected cell lysate (TC) using NB 100-454.

Immunohistochemistry-Paraffin: VG5Q Antibody [NB100-454] - VG5Q was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human VG5Q polyclonal Antibody (Catalog # NB100-454) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in hepatocytes.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

VG5Q Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit VG5Q Antibody - Azide and BSA Free (NB100-454) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. Anti-VG5Q Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide mapping to an epitope within residues 500-600 of the human VG5Q protein. [UniProt# Q8N302]
• Localization: Cytoplasm
• Specificity: This is specific for human VG5Q.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AGGF1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA reported in scientific literature
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation reported in scientific literature
  • Western Blot 1:500
• Application Notes: This VG5Q antibody can be used for Immunohistochemistry and Western Blot, where a specific band is seen at 100 kDa. Other bands are non-specific or a degradation product (~75 kDa). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for VG5Q Antibody - Azide and BSA Free

• AGGF1
• Angiogenic factor VG5Q
• angiogenic factor with G patch and FHA domains 1
• FLJ10283hVG5Q
• GPATC7G patch domain-containing protein 7
• GPATCH7HUS84971
• HSU84971
• VG5Q
• VG5QVasculogenesis gene on 5q protein

Background

VG5Q (vasculogenesis gene on 5q protein, also called AGGF1) is a novel angiogenic factor that has been identified through genetic analysis of Klippel-Trenaunay syndrome (KTS), a congenital vascular disorder composed of capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. VG5Q is broadly expressed in different cell types including endothelial cells (umbilical vein endothelial cells and microvascular endothelial cells), vascular smooth muscle cells and osteoblasts. VG5Q expression is mainly cytoplasmic and upon angiogenic stimulation especially when endothelial cell tube formation is initiated, VG5Q gets secreted and interact with a secreted angiogenic factor TNFSF12. VG5Q promotes angiogenesis as well as the proliferation of endothelial cells through direct binding in an autocrine fashion. Moreover, it has been demonstrated to promote angiogenesis as potentially as VEGF and its expression decreases with knockdown of GATA1 gene which is proposed as VG5Q regulator through two GATA1 binding sites in VG5Q promoter region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VG5Q Antibody (NB100-454)(1)

Publication using NB100-454

• Tian, XL, Kadaba, R et al. Identification of an angiogenic factor that when mutated causes susceptibility to Klippel-Trenaunay syndrome. Nature;427(6975):640-5. 2004-02-12 [PMID: 14961121] (ICC/IF, WB, IP, ELISA, Human)

Bioinformatics

• Gene Symbol: AGGF1
• Entrez:
  • 55109(Human)
• Uniprot:
  • Q8N302()