VG5Q Antibody

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Western Blot: VG5Q Antibody [NB100-454] - Human VG5Q detected in HT1080 non-transfected cell lysate (NTC) and HT1080 transfected cell lysate (TC) using NB 100-454.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

VG5Q Antibody Summary

Immunogen
A synthetic peptide mapping to an epitope within residues 500-600 of the human VG5Q protein. [UniProt# Q8N302]
Localization
Cytoplasm
Specificity
This is specific for human VG5Q.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AGGF1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:250
  • Immunoprecipitation
Application Notes
This VG5Q antibody can be used for Immunohistochemistry and Western Blot, where a specific band is seen at 100 kDa. Other bands are non-specific or a degradation product (~75 kDa). Immunocytochemistry/Immunofluorescence, ELISA and Immunoprecipitation were reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB100-454 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for VG5Q Antibody

  • AGGF1
  • Angiogenic factor VG5Q
  • angiogenic factor with G patch and FHA domains 1
  • FLJ10283hVG5Q
  • GPATC7G patch domain-containing protein 7
  • GPATCH7HUS84971
  • HSU84971
  • VG5Q
  • VG5QVasculogenesis gene on 5q protein

Background

VG5Q (vasculogenesis gene on 5q protein, also called AGGF1) is a novel angiogenic factor that has been identified through genetic analysis of Klippel-Trenaunay syndrome (KTS), a congenital vascular disorder composed of capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. VG5Q is broadly expressed in different cell types including endothelial cells (umbilical vein endothelial cells and microvascular endothelial cells), vascular smooth muscle cells and osteoblasts. VG5Q expression is mainly cytoplasmic and upon angiogenic stimulation especially when endothelial cell tube formation is initiated, VG5Q gets secreted and interact with a secreted angiogenic factor TNFSF12. VG5Q promotes angiogenesis as well as the proliferation of endothelial cells through direct binding in an autocrine fashion. Moreover, it has been demonstrated to promote angiogenesis as potentially as VEGF and its expression decreases with knockdown of GATA1 gene which is proposed as VG5Q regulator through two GATA1 binding sites in VG5Q promoter region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VG5Q Antibody (NB100-454)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA, ICC/IF, IP, WB.


Filter By Application
ELISA
(1)
ICC/IF
(1)
IP
(1)
WB
(1)
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Human
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AGGF1
Entrez
Uniprot