Ras-GAP Antibody

Images

 
Western blot shows lysates of TT human medullary thyroid cancer cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Ras-GAP Antibody Summary

Immunogen
E. coli-derived recombinant human Ras-GAP
Trp181-Val272
Accession # P20936
Specificity
Detects endogenous human, mouse, and rat Ras-GAP in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RASA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF5094 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ras-GAP Antibody

  • CM-AVM
  • PKWS
  • RASA1
  • RasGAP
  • Ras-GAP

Background

Ras-GAP (Ras GTPase-activating protein) interacts with Ras to stimulate the otherwise weak intrinsic GTPase activity of Ras and to promote the return of Ras to an inactive GDP-bound state. Thus, Ras-GAP is a negative regulator of Ras. Human Ras-GAP is 1047 amino acids in length. The N-terminal region contains two SH2 domains and an intervening SH3 domain, while the C-terminal half of Ras-GAP is sufficient to accelerate Ras GTPase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ras-GAP Antibody (AF5094)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RASA1
Entrez
Uniprot