SynGAP Antibody

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Immunocytochemistry/ Immunofluorescence: SynGAP Antibody [NB120-3344] - Immunofluorescence analysis of SynGAP was performed using 70% confluent log phase PC-12 cells.
Immunohistochemistry-Paraffin: SynGAP Antibody [NB120-3344] - Immunohistochemistry analysis of SYNGAP showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control ...read more
Immunocytochemistry/ Immunofluorescence: SynGAP Antibody [NB120-3344] - Rat hippocampal neurons
Immunocytochemistry/ Immunofluorescence: SynGAP Antibody [NB120-3344]

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SynGAP Antibody Summary

Immunogen
GST fusion protein of a recombinant fragment, residues 947-1167, of rat SynGAP.
Specificity
This detects synaptic Ras-GTPase activating protein (SynGAP) from mouse and rat tissues.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYNGAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Immunocytochemistry: use at a concentration of 1 ug/ml.Western Blot: use at a concentration of 1 ug/ml.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~135 kDa protein representing SynGAP from mouse brain tissue homogenate. Immunocytochemical staining of SynGAP in rat hippocampal neurons yields a pattern consistent with vesicular staining.

Reactivity Notes

Cross-reacts with Rat and Mouse.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SynGAP Antibody

  • DKFZp761G1421
  • KIAA1938135kDa
  • MRD5
  • synaptic Ras GTPase activating protein 1 homolog (rat)
  • synaptic Ras GTPase activating protein 1
  • Synaptic Ras-GAP 1

Background

Synaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SYNGAP1
Entrez