Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

ELISA: Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric) [NBP2-78888] - Samples were spiked with high concentrations of Rat VEGFR2/KDR/Flk-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric) [NBP2-78888] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, and other biological fluids
• Standard Curve Range: 0.16-10 ng/mL
• Sensitivity: 0.10 ng/mL

Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat VEGFR-2/KDR in samples. No Significant cross-reactivity or interference between Rat VEGFR-2/KDR and analogues was observed.
• Standard Curve Range: 0.16-10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.78%
• Intra-Assay: CV% < 5.39%
• Spike Recovery: 86-108%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: KDR

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

• CD309 antigen
• CD309
• EC 2.7.10
• EC 2.7.10.1
• Fetal liver kinase 1
• fetal liver kinase-1
• Flk1
• Flk-1
• FLK1tyrosine kinase growth factor receptor
• KDR
• kinase insert domain receptor (a type III receptor tyrosine kinase)
• Kinase insert domain receptor
• KRD1
• Ly73
• Protein-tyrosine kinase receptor flk-1
• soluble VEGFR2
• vascular endothelial growth factor receptor 2
• VEGF R2
• VEGFR
• VEGFR2
• VEGFR-2

Background

Vascular endothelial growth factor receptor 2 (VEGFR2) is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other common growth factor receptors, VEGF receptor 2 dimerises upon ligand binding and is autophosphorylated at multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or can serve as binding sites for SH2 and phosphotyrosine binding containing signaling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.

Defects in the VEGFR2 gene are associated with susceptibility to benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium called hemangioma capillary infantile. HCI is the most common tumor found in infants, found in up to 10% of all births.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for VEGFR2/KDR/Flk-1 ELISA Kit (NBP2-78888). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: KDR
• Uniprot:
  • O08775(Rat)
  • O08775(Rat)