VEGFR2/KDR/Flk-1 Antibody (20I6) [Alexa Fluor® 488]

Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) [Alexa Fluor® 488] [NBP1-18646AF488] - HMEC-1 CRL-3243 cells, stained with 1:50 VEGFR2/KDR/Flk-1 antibody (NBP1-18646AF488) (green) and DAPI (blue). Image from verified customer review.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 20I6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

VEGFR2/KDR/Flk-1 Antibody (20I6) [Alexa Fluor® 488] Summary

• Immunogen: Recombinant human soluble extracellular KDR (D7)
• Marker: Endothelial Cell Marker
• Specificity: The monoclonal antibody will detect native human VEGFR-2/KDR in ELISA experiments and on the surface of different human cell types.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KDR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody (20I6) [Alexa Fluor® 488]

• CD309 antigen
• CD309
• EC 2.7.10
• EC 2.7.10.1
• Fetal liver kinase 1
• fetal liver kinase-1
• Flk1
• Flk-1
• FLK1tyrosine kinase growth factor receptor
• KDR
• kinase insert domain receptor (a type III receptor tyrosine kinase)
• Kinase insert domain receptor
• KRD1
• Ly73
• Protein-tyrosine kinase receptor flk-1
• soluble VEGFR2
• vascular endothelial growth factor receptor 2
• VEGF R2
• VEGFR
• VEGFR2
• VEGFR-2

Background

Vascular endothelial growth factor receptor 2 (VEGFR2) is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other common growth factor receptors, VEGF receptor 2 dimerises upon ligand binding and is autophosphorylated at multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or can serve as binding sites for SH2 and phosphotyrosine binding containing signaling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.

Defects in the VEGFR2 gene are associated with susceptibility to benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium called hemangioma capillary infantile. HCI is the most common tumor found in infants, found in up to 10% of all births.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for VEGFR2/KDR/Flk-1 Antibody (NBP1-18646AF488) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Jernej Vajda ICC Human 04/26/2022

Summary

• Application: Immunocytochemistry
• Sample Tested: HMEC-1 CRL-3243,HUVEC PCS-100-010
• Species: Human
• Lot: D101746

Comments

• Comments: The cells were stained with a 1:50 dilution, even though the unconjugated version of this antibody has a protocol for 1:80 dilution.

Bioinformatics

• Gene Symbol: KDR