VEGFR1/Flt-1 Antibody (EWF) - Azide and BSA Free

Western Blot: VEGFR1/Flt-1 Antibody (EWF) [NB600-1006] - VEGF Receptor 1 Antibody (EWF) [NB600-1006] - The monoclonal anti-human VEGFR-1/Flt-1 cross reacts with recombinant murine sFlt-1.

Flow Cytometry: VEGFR1/Flt-1 Antibody (EWF) [NB600-1006] - analysis with porcine aortic endothelial cells expressing human VEGFR-1/Flt-1.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IP, CyTOF-ready
• Clone: EWF
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

VEGFR1/Flt-1 Antibody (EWF) - Azide and BSA Free Summary

• Description: PLEASE NOTE: 0.02mg size is provided in liquid format.
• Immunogen: Recombinant human soluble Flt-1 (D5)
• Localization: Type I membrane protein (Flt1) and soluble (SFlt1).
• Marker: Endothelial Cell Marker
• Specificity: This will recognise the recombinant and naturally occurring form of human VEGFR1/Flt1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1-10 ug/ml
  • Flow Cytometry
  • Immunoprecipitation 1-5 ug/ml lysate
  • Western Blot 1-10 ug/ml
• Application Notes: WB: Predicted molecular weight: 150 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Alternate Names for VEGFR1/Flt-1 Antibody (EWF) - Azide and BSA Free

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

Receptors for VEGF, VEGFB and PGF have tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors--the tyrosine kinases FLK1 and FLT1--are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR1/Flt-1 Antibody (NB600-1006)(1)

Publication using NB600-1006

• Jesus ANB, Taha A, Wang D et al. Increased Expression of the ?133p53? Isoform Enhances Brain Metastasis International Journal of Molecular Sciences 2023-01-09 [PMID: 36674782] (Flow Cytometry)

Bioinformatics

• Gene Symbol: FLT1
• Entrez:
  • 2321(Human)
• Uniprot:
  • P17948()