VEGFR1/Flt-1 Antibody (EWF)

Images

 
Western Blot: VEGFR1/Flt-1 Antibody (EWF) [NB600-1006] - VEGF Receptor 1 Antibody (EWF) [NB600-1006] - The monoclonal anti-human VEGFR-1/Flt-1 cross reacts with recombinant murine sFlt-1.
ELISA: VEGF Receptor 1 Antibody (EWF) [NB600-1006] - Cross-reactivity of monoclonal and polyclonal anti-human Flt-1 antibodies with mouse Flt-1 Standard ELISA assay with coating of 2.5 ug/ml mouse sFlt-1-Fc

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
EWF
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

VEGFR1/Flt-1 Antibody (EWF) Summary

Description
PLEASE NOTE: 0.02mg size is provided in liquid format.
Immunogen
Recombinant human soluble extracellular Flt-1 D1-D5 was used as the immunizing antigen.
Localization
Type I membrane protein (Flt1) and soluble (SFlt1).
Marker
Endothelial Cell Marker
Specificity
This will recognise the recombinant and naturally occurring form of human VEGFR1/Flt1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FLT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • ELISA 1.5-8 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1-5 ug/ml lysate
Application Notes
WB: Predicted molecular weight: 150 kDa. Optimal dilutions/concentrations should be determined by the end user.
Positive Control
VEGFR1/Flt-1 Lysate (NBL1-06699)

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
No Preservative
Purity
Protein G purified
Reconstitution Instructions
Please note the larger size(s) come in lyophilized form and the sample size will be in liquid formate. Reconstitute with sterilized water to a final concentration of 0.1 - 1.0 mg/ml.

Notes

PLEASE NOTE: 0.02mg size is provided in liquid format.

Alternate Names for VEGFR1/Flt-1 Antibody (EWF)

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VEGFR1/Flt-1 Antibody (NB600-1006) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-1006AF405
Alexa Fluor 488 Labeled NB600-1006AF488
Alexa Fluor 647 Labeled NB600-1006AF647
Alexa Fluor 700 Labeled NB600-1006AF700
DyLight 350 Labeled NB600-1006UV
DyLight 405 Labeled NB600-1006V
DyLight 488 Labeled NB600-1006G
DyLight 680 Labeled NB600-1006FR
DyLight 755 Labeled NB600-1006IR

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Bioinformatics Tool for VEGFR1/Flt-1 Antibody (NB600-1006)

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Bioinformatics

Gene Symbol FLT1
Entrez
Uniprot