VEGF Antibody (26503) [Unconjugated]

Images

 
Western blot shows 25 ng of Recombinant Human VEGF165 (Catalog # 293‑VE), Recombinant Human VEGF111 (Catalog # 5336-VE), Recombinant Human VEGF121, aa 207-327 (Catalog # 4644-VS), Recombinant Human VEGF145 (aa ...read more
Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
26503
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price

VEGF Antibody (26503) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165
Ala27-Arg191
Accession # NP_001165097.1
Specificity
Detects human VEGF165 and human VEGF121 in ELISAs and Western blots. In ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) VEGF and recombinant rat (rr)VEGF and no cross-reactivity with recombinant human (rh) VEGF-D is obseved.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
VEGFA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 10-60 ng/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human VEGF165 Biotinylated Antibody (Catalog number BAF293)
Standard: Recombinant Human VEGF165 (Catalog number 293-VE)
Publications
Read Publications using
MAB293 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF Antibody (26503) [Unconjugated]

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an approximately 44 kDa molecular weight homodimer sharing 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

233-FB/CF
Species: Hu
Applications: BA
AF321
Species: Hu
Applications: Block, CyTOF-ready, Flow, IHC, WB
AF644
Species: Mu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
NB100-105
Species: Bv, Ca, Fe, Ma, Hu, Pm, Mu, Po, Pm, Rb, Rt, Sh, Xp
Applications: ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, LA, PLA, TCS, WB
D6050
Species: Hu
Applications: ELISA
923-AN/CF
Species: Hu
Applications: BA
AF3628
Species: Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, WB
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NB110-41083
Species: Hu
Applications: ChIP, ELISA, ICC/IF, WB
NB600-1071
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
DPG00
Species: Hu
Applications: ELISA
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
294-HG/CF
Species: Hu
Applications: BA

Publications for VEGF Antibody (MAB293)(55)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, Complex Species Category, Porcine, Primate.

We have publications tested in 10 applications: Array Development, ELISA Development, ELISA Microarray Development, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Array Development
(1)
ELISA Development
(2)
ELISA Microarray Development
(1)
Flow Cytometry
(1)
ICC
(3)
IHC
(2)
IHC-Fr
(1)
IHC-P
(5)
Neutralization
(23)
Western Blot
(15)
All Applications
Filter By Species
Human
(43)
Mouse
(5)
Rat
(2)
Bovine
(1)
Complex Species Category
(1)
Porcine
(2)
Primate
(1)
All Species
Showing Publications 1 - 10 of 55. Show All 55 Publications.
Publications using MAB293 Applications Species
AM Abu El-Asr, MI Nawaz, A Ahmad, MM Siddiquei, E Allegaert, PW Gikandi, G De Hertogh, G Opdenakker CD146/Soluble CD146 Pathway Is a Novel Biomarker of Angiogenesis and Inflammation in Proliferative Diabetic Retinopathy Investigative Ophthalmology & Visual Science, 2021;62(9):32. 2021 [PMID: 34293080] (Western Blot, Human) Western Blot Human
KV Manian, CA Galloway, S Dalvi, AA Emanuel, JA Mereness, W Black, L Winschel, C Soto, Y Li, Y Song, W DeMaria, A Kumar, I Slukvin, MP Schwartz, WL Murphy, B Anand-Apte, M Chung, DSW Benoit, R Singh 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration Cell Stem Cell, 2021;0(0):. 2021 [PMID: 33784497] (ICC, Human) ICC Human
ZY Liao, IC Liang, HJ Li, CC Wu, HM Lo, DC Chang, CF Hung Chrysin Inhibits High Glucose-Induced Migration on Chorioretinal Endothelial Cells via VEGF and VEGFR Down-Regulation Int J Mol Sci, 2020;21(15):. 2020 [PMID: 32748894] (Western Blot, Primate) Western Blot Primate
HA Oh, J Kwak, BJ Kim, HJ Jin, WS Park, SJ Choi, W Oh, S Um Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth Cells, 2020;9(6):. 2020 [PMID: 32481584] (Neutralization, Human) Neutralization Human
DJ Perdicaro, C Rodriguez, J Gambarte T, RM Miatello, PI Oteiza, MA Vazquez Pr Quercetin attenuates adipose hypertrophy, in part through activation of adipogenesis in rats fed a high-fat diet J. Nutr. Biochem., 2020;79(0):108352. 2020 [PMID: 32145471] (Western Blot, Rat) Western Blot Rat
AF Stassi, F Gasser, MML Velázquez, EM Belotti, NC Gareis, F Rey, HH Ortega, NR Salvetti, ME Baravalle Contribution of the VEGF system to the follicular persistence associated with bovine cystic ovaries Theriogenology, 2019;138(0):52-65. 2019 [PMID: 31301448] (Western Blot, Bovine) Western Blot Bovine
AF Stassi, F Gasser, MML Velázquez, EM Belotti, NC Gareis, F Rey, HH Ortega, NR Salvetti, ME Baravalle Contribution of the VEGF system to the follicular persistence associated with bovine cystic ovaries Theriogenology, 2019;138(0):52-65. 2019 [PMID: 31301448] (Western Blot, Mouse) Western Blot Mouse
J Ren, C Gu, Y Yang, J Xue, Y Sun, F Jian, D Chen, L Bian, Q Sun TSP-1 is downregulated and inversely correlates with miR-449c expression in Cushing&#039;s disease J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 31016850] (Western Blot, Mouse) Western Blot Mouse
P Zizza, R Dinami, M Porru, C Cingolani, E Salvati, A Rizzo, C D'Angelo, E Petti, CA Amoreo, M Mottolese, I Sperduti, A Chambery, R Russo, P Ostano, G Chiorino, G Blandino, A Sacconi, J Cherfils-V, C Leonetti, E Gilson, A Biroccio TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment Nucleic Acids Res., 2019;0(0):. 2019 [PMID: 30698737] (Neutralization, Human) Neutralization Human
HK Kim, SG Lee, SW Lee, BJ Oh, JH Kim, JA Kim, G Lee, JD Jang, YA Joe A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells Stem Cells, 2018;0(0):. 2018 [PMID: 30281870] (Neutralization, Human) Neutralization Human
Show All 55 Publications.

Reviews for VEGF Antibody (MAB293) (0)

There are no reviews for VEGF Antibody (MAB293). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VEGF Antibody (MAB293). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional VEGF Products

Bioinformatics Tool for VEGF Antibody (MAB293)

Discover related pathways, diseases and genes to VEGF Antibody (MAB293). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for VEGF Antibody (MAB293)

Discover more about diseases related to VEGF Antibody (MAB293).
 

Pathways for VEGF Antibody (MAB293)

View related products by pathway.

PTMs for VEGF Antibody (MAB293)

Learn more about PTMs related to VEGF Antibody (MAB293).

Blogs on VEGF. Showing 1-10 of 22 blog posts - Show all blog posts.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit.  ...  Read full blog post.

Showing 1-10 of 22 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VEGF Antibody (26503) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol VEGFA
Uniprot