Western blot shows 25 ng of Recombinant Human VEGF165 (Catalog # 293‑VE), Recombinant Human VEGF111 (Catalog # 5336-VE), Recombinant Human VEGF121, aa 207-327 (Catalog # 4644-VS), Recombinant Human VEGF145 (aa ...read more
Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation ...read more
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165 Ala27-Arg191 Accession # NP_001165097.1
Detects human VEGF165 and human VEGF121 in ELISAs and Western blots. In ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) VEGF and recombinant rat (rr)VEGF and no cross-reactivity with recombinant human (rh) VEGF-D is obseved.
Protein A or G purified from hybridoma culture supernatant
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for VEGF Antibody (26503) [Unconjugated]
vascular endothelial growth factor A
Vascular permeability factor
VPFvascular endothelial growth factor
Vascular Endothelial Growth Factor (VEGF or VEGF-A), also
known as Vascular Permeability Factor (VPF), is a potent mediator of both
angiogenesis and vasculogenesis in the fetus and adult. It is a member of the
PDGF family that is characterized by the presence of eight conserved cysteine
residues and a cystine knot structure. VEGF165 appears to be the most abundant
and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an
approximately 44 kDa molecular weight homodimer sharing 88% aa sequence
identity with corresponding regions of mouse and rat, 96% with porcine, 95%
with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF
binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called
Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is
highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of
VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1
and promotes complex formation with VEGF R2. VEGF is required during
embryogenesis and functions to regulate the proliferation, migration, and
survival of endothelial cells. In adults, VEGF functions mainly in wound
healing and the female reproductive cycle. Pathologically, it is involved in
tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with
disease activity in autoimmune diseases such as rheumatoid arthritis, multiple
sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and
cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for VEGF Antibody (MAB293). (Showing 1 - 1 of 1 FAQs).
Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
VEGF: Vascular endothelial growth factor VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g... Read full blog post.
LYVE1 - It's Alive! It's a LYVE LYVE1 (lymphatic vessel endothelial hyaluronan receptor-1) is one of the most specific and widely used mammalian lymphatic endothelial markers.It is found in lymph nodes and at the luminal/abluminal surfaces of lymphatic vessels. It is a single-pass t... Read full blog post.
LOX: A prime enzyme LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh... Read full blog post.
VEGF Receptors, Angiogenesis and Cancer Vascular endothelial growth factor receptors 1 and 2 (VEGFR1 and VEGFR2) are related family members of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. They are key regulators of physiological angiogenesis du... Read full blog post.
Controlling the HIF-1 Switch Hypoxia-inducible factor-1 is a major transcription factor composed of two subunits: HIF-1alpha and HIF-1 beta. Under normoxic conditions, HIF-1 alpha is targeted to proteosomal degradation via ubiquitination. On the other hand during hypoxic conditio... Read full blog post.
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