VEGF Antibody (211615) [Unconjugated]

Recombinant Zebrafish VEGF165 (Catalog # 1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF165 (100 ng/mL) is neutral­lized (green line) by increasing concentrations of Mouse Anti-Zebrafish VEGF Monoclonal Antibody (Catalog # MAB1247). The ND50 is typically 0.2‑0.5 µg/mL.

• Reactivity: Ze
• Applications: WB, B/N
• Clone: 211615
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VEGF Antibody (211615) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant zebrafish VEGF₁₆₅
  Ala24-Arg188
  Accession # O73682.1
• Specificity: Detects zebrafish VEGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) VEGF₁₂₁, ₁₆₅, ₂₀₆, rhVEGF-B₁₈₆, rhVEGF-C, rhVEGF‑D, recombinant mouse (rm) VEGF₁₁₅, rmVEGF₁₆₅, rmVEGF-B₁₈₆, rmVEGF‑D, or recombinant rat VEGF₁₆₄ is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: vegfaa
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.2-0.5 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF Antibody (211615) [Unconjugated]

• MVCD1
• VAS
• vascular endothelial growth factor A
• Vascular permeability factor
• Vasculotropin
• VEGF
• VEGFA
• VEGF-A
• VEGFMGC70609
• VPF
• VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternate splice isoforms of VEGF-A, ranging from 206 amino acids (aa) to 121 aa in length, are known. In zebrafish, two VEGF isoforms, a 165 aa and a 121 aa isoform, have been reported. Mature zebrafish VEGF₁₆₅ shares 64%, 62% and 62% aa sequence identity with frog, human, and mouse VEGF₁₆₅, respectively. There are two tyrosine kinase receptors for VEGF reported in mammals termed VEGF R1 and VEGF R2/FLK-1. One receptor has been identified in zebrafish (FLK-1), and this may actually represent the orthologue to the early common ancestor for mammalian VEGF R1 and R2. All receptors are type I transmembrane proteins that show seven immunoglobulin-like domains extracellularly and a split kinase domain intracellularly. In addition to the tyrosine kinase receptors, neuropilin-1 (NRP-1) has been reported to be a coreceptor for VEGF binding. It is proposed that the presence of NRP-1 lowers the concentration of VEGF necessary for activation of VEGF R2. NRP-1 has been reported in both zebrafish and human. VEGF regulates multiple biological functions in endothelial cells, including cell proliferation, migration and survival. These functions of VEGF are mediated partly through the induction of nitric oxide and prostacyclin, as well as upregulation of metalloproteinases. Together with other vascular-specific growth factors such as the Angiopoietins, VEGF have separate but complementary roles in angiogenesis and vasculogenesis (1‑7).

1. Laing, D. et al. (1998) Biochem. Biophys. Acta 1397:14.
2. Laing, D. and R. Ge (1998) GenBank Accession # AAC14713.
3. Laio, W. et al. (1997) Development 124:381.
4. Lee, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10470.
5. Thurston, G. (2002) J. Anat. 200:575.
6. Zachary, I. and G. Gliki (2001) Cardiovasc. Res. 49:568.
7. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGF Antibody (MAB1247)(4)

Publications using MAB1247

• Robyn A. Umans, Carolyn Pollock, William A. Mills, Kareem C. Clark, Y. Albert Pan, Harald Sontheimer Using Zebrafish to Elucidate Glial-Vascular Interactions During CNS Development Frontiers in Cell and Developmental Biology 2021-06-29 [PMID: 34268301]
• J Müller-Dei, P Schröder, L Beverly-St, R Hiss, J Fiedler, J Nyström, T Thum, H Haller, M Schiffer Overexpression of preeclampsia induced microRNA-26a-5p leads to proteinuria in zebrafish Sci Rep, 2018-02-26;8(1):3621. 2/26/2018 [PMID: 29483572]
• Maeda Y, Suzuki T, Pan X, Chen G, Pan S, Bartman T, Whitsett JA CUL2 is required for the activity of hypoxia-inducible factor and vasculogenesis. J. Biol. Chem., 2008-03-27;283(23):16084-92. 2008-03-27 [PMID: 18372249] (Western Blot, Zebrafish)
• Vigneswaran N, Sacks P, Gilcrease M, Zacharias W Microarray gene expression profiling of cell lines from primary and metastatic tongue squamous cell carcinoma: possible insights from emerging technology. J. Oral Pathol. Med., 2005-02-01;34(2):77-86. 2005-02-01 [PMID: 15641986] (ICC, Human)

FAQs for VEGF Antibody (MAB1247). (Showing 1 - 1 of 1 FAQs).

1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
   • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Bioinformatics

• Gene Symbol: vegfaa
• Uniprot:
  • O73682.1()