VE-Cadherin [p Tyr731] Antibody - BSA Free

Western Blot: VE-Cadherin [p Tyr731] Antibody [NBP2-54766] - Analysis of extracts from HepG2 cells treated with Na3VO4 using Phospho-VE-Cadherin (Tyr731) Antibody. The lane on the right is treated with the antigen-specific peptide.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

VE-Cadherin [p Tyr731] Antibody - BSA Free Summary

• Immunogen: VE-Cadherin (phospho Tyr731) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine731 (H-I-Y (p)-G-Y) derived from Human VE-Cadherin.
• Modification: p Tyr731
• Specificity: The antibody detects endogenous level of VE-Cadherin only when phosphorylated at tyrosine 731.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDH5
• Purity: Epitope affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Epitope affinity purified

Alternate Names for VE-Cadherin [p Tyr731] Antibody - BSA Free

• 7B4 antigen
• 7B4
• cadherin 5, type 2 (vascular endothelium)
• cadherin 5, type 2, VE-cadherin (vascular epithelium)
• Cadherin-5
• CD144 antigen
• CD144
• CDH5
• endothelial-specific cadherin
• FLJ17376
• Vascular endothelial cadherin
• VECadherin
• VE-Cadherin

Background

VE-cadherin (cadherin-5) is an endothelial specific adhesion molecule that is essential for the maintenance of endothelial barrier function and angiogenesis. VE-cadherin is linked to the actin cytoskeleton through a number of adaptor proteins including a-, â-, and ã-catenin. Cytoskeletal dynamics and phosphorylation regulate VE-cadherin mediated cell-cell adhesion. The cytosolic C-terminal Src kinase (Csk) binds via its SH2 domain to the phosphorylated tyrosine 658 site of VE-cadherin. This interaction regulates density dependent cell proliferation. Phosphorylation of tyrosine 658 has also been shown to lead to the uncoupling of p120-catenin from the cytoplasmic tail of VE-cadherin. This phosphorylation event is sufficient to maintain cells in a mesenchymal state during the cell invasion phase of angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VE-Cadherin Antibody (NBP2-54766)(1)

Publication using NBP2-54766

• Y Takagaki, SM Lee, Z Dongqing, M Kitada, K Kanasaki, D Koya Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis Autophagy, 2020-01-22;0(0):1-10. 2020-01-22 [PMID: 31965901]

Bioinformatics

• Gene Symbol: CDH5