VE-Cadherin Antibody (BV13) [Biotin]

Flow Cytometry: VE-Cadherin Antibody (BV13) [Biotin] [NBP1-43579] - Staining of bEnd.3 cell line with 0.5 micrograms of Rat IgG1 k Isotype Control Biotin (blue histogram) or 0.125 micrograms of Anti-Mouse CD144 (VE-Cadherin) Biotin purple histogram) followed by Streptavidin PE. Total viable cells were used for analysis.

• Reactivity: Mu
• Applications: WB, Flow, IHC
• Clone: BV13
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: 0.5 mg/ml

VE-Cadherin Antibody (BV13) [Biotin] Summary

• Immunogen: The immunogen for this antibody was CD144.
• Marker: Endothelial Cell Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CDH5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Intravenous injection of BV13 has been shown to induce a concentration- and timedependent increase in vascular permeability in the heart and lungs.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for VE-Cadherin Antibody (BV13) [Biotin]

• 7B4 antigen
• 7B4
• cadherin 5, type 2 (vascular endothelium)
• cadherin 5, type 2, VE-cadherin (vascular epithelium)
• Cadherin-5
• CD144 antigen
• CD144
• CDH5
• endothelial-specific cadherin
• FLJ17376
• Vascular endothelial cadherin
• VECadherin
• VE-Cadherin

Background

The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDH5