Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric)

ELISA: Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric) [NBP2-82389] - Samples were spiked with high concentrations of Human VDR/NR1I1/Vitamin D Receptor and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric) [NBP2-82389] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL

Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human VDR/NR1I1/Vitamin D Receptor in samples. No Significant cross-reactivity or interference between Human VDR/NR1I1/Vitamin D Receptor and analogues was observed.
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.27
• Intra-Assay: CV% < 5
• Spike Recovery: 85-102%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: VDR

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric)

• NR1I1
• NR1I1Nuclear receptor subfamily 1 group I member 11,25-dihydroxyvitamin D3 receptor
• VDR
• vitamin D1,25- dihydroxyvitamin D3 receptor
• vitamin D3 receptor

Background

Vitamin D Receptor encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for VDR/NR1I1/Vitamin D Receptor ELISA Kit (NBP2-82389). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VDR