CYP24A1 Antibody

Images

 
Orthogonal Strategies: Western Blot: CYP24A1 Antibody [NBP1-85495] - Analysis in human cell lines A-549 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Immunocytochemistry/ Immunofluorescence: CYP24A1 Antibody [NBP1-85495] - Immunofluorescent staining of human cell line A549 shows localization to mitochondria. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-85495] - Staining in human urinary bladder and skeletal muscle tissues using anti-CYP24A1 antibody. Corresponding CYP24A1 RNA-seq data ...read more
Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-85495] - Staining of human urinary bladder shows high expression
Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-85495] - Staining of human skeletal muscle shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

CYP24A1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DFLCDIYHQNRLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQVQQKLLKEIQSVLPENQVPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKATVLGEYALPKGTVLMLNTQV
Specificity
Specificity of human CYP24A1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (91%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP24A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CYP24A1 Protein (NBP1-85495PEP)
Publications
Read Publication using NBP1-85495.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23334593)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CYP24A1 Antibody

  • 25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
  • CP241
  • CYP241,25-alphadihydroxyvitamin D3 24-hydroxylase
  • Cytochrome P450 24A1
  • cytochrome P450, family 24, subfamily A, polypeptide 1,24-OHase
  • cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
  • Cytochrome P450-CC24
  • EC 1.14.13.n4
  • exo-mitochondrial protein
  • MGC126273
  • MGC126274
  • P450-CC24
  • vitamin D 24-hydroxylase
  • Vitamin D(3) 24-hydroxylase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CYP24A1 Antibody (NBP1-85495)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CYP24A1