Recombinant Human CYP27B1 GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, MA, AP

Recombinant Human CYP27B1 GST (N-Term) Protein Summary

• Description: A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 409-508 of Human CYP27B1
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: TLCHYATSRDPAQFPEPNSFRPARWLGEGPTPHPFASLPFGFGKRSCMGRRLAELELQMALAQILTHFEVQPEPGAAPVRPKTRTVLVPERSINLQFLDR

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Partial Recombinant Protein
• Gene: CYP27B1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 36.74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human CYP27B1 GST (N-Term) Protein

• 1alpha(OH)ase
• 25 hydroxyvitamin D3-1-alpha hydroxylase
• 25-hydroxyvitamin D(3) 1-alpha-hydroxylase
• 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
• 25-OHD-1 alpha-hydroxylase
• Calcidiol 1-monooxygenase
• CP2B
• CYP1
• CYP1ALPHA
• CYP27B
• CYP27B1
• Cytochrome p450 27B1
• Cytochrome P450 subfamily XXVIIB polypeptide 1
• cytochrome P450, family 27, subfamily B, polypeptide 1
• cytochrome P450, subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase)
• Cytochrome P450C1 alpha
• Cytochrome P450VD1-alpha
• EC 1.14.13.13
• EC 1.14.15.18
• P450c1
• PDDR
• polypeptide 1
• VD3 1A hydroxylase
• VDD1
• VDDR
• VDDRI
• VDR

Background

CYP27B1 - cytochrome P450, family 27, subfamily B, polypeptide 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP27B1