VDR/NR1I1/Vitamin D Receptor Antibody

Product Discontinued

VDR/NR1I1/Vitamin D Receptor Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 11-60 of Human VDR.
• Specificity: VDR (K45) pAb detects endogenous levels of VDR protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VDR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of VDR (K45) pAb in extracts from Jurkat cells.
  Immunohistochemistry (IHC) analyzes of VDR (K45) pAb in paraffin-embedded human breast carcinoma tissue. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27548154).
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VDR/NR1I1/Vitamin D Receptor Antibody

• NR1I1
• NR1I1Nuclear receptor subfamily 1 group I member 11,25-dihydroxyvitamin D3 receptor
• VDR
• vitamin D1,25- dihydroxyvitamin D3 receptor
• vitamin D3 receptor

Background

The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally-specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9- cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VDR/NR1I1/Vitamin D Receptor Antibody (NBP1-51322)(4)

Publications using NBP1-51322

• Okrit F, Chantranuwatana P, Werawatganon D, et al. Changes of vitamin D receptors (VDR) and MAPK activation in cytoplasmic and nuclear fractions following exposure to cigarette smoke with or without filter in rats Heliyon 2021-01-01 [PMID: 33553726] (WB, IF/IHC, Rat)
• Nakhoul N, Thawko T, Farber E et al. The Therapeutic Effect of Active Vitamin D Supplementation in Preventing the Progression of Diabetic Nephropathy in a Diabetic Mouse Model Journal of Diabetes Research 2020-11-26 [PMID: 33294462] (WB, Mouse)
• El-Hanbuli HM, Dawoud NM, Mahmoud RH. Narrow-band UVB effects on cutaneous vitamin D receptor expression and serum 25-hydroxyvitamin D in generalized vitiligo Photodermatol Photoimmunol Photomed. 2017-10-28 [PMID: 29080365] (IF/IHC, Human)
• Hou YF, Gao SH, Wang P et al. 1alpha, 25 (OH) 2D3 Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial-Mesenchymal Transition. Int J Mol Sci. 2016-08-19 [PMID: 27548154] (ICC/IF, WB, Human)

Bioinformatics

• Gene Symbol: VDR