VCAM-1/CD106 Antibody (MR106) - BSA Free

Western Blot: VCAM-1/CD106 Antibody (MR106) [NBP1-43368] - Western blot analyses of protein expressions of glutathione peroxidase-1 (GPx1), vascular cell adhesion molecule 1 (VCAM-1), inducible nitric oxide (iNOS), nuclear factor (NF-kappaB), matrix metalloproteinase-9 (MMP-9), and surfactant protein A and D (SP-A and SP-D) in lung (n = 3; * and # signify P < 0.05 and P < 0.001, respectively) Image collected and cropped by CiteAb from the following publication (//bmcpulmmed.biomedcentral.com/articles/10.1186/s12890-017-0448-9), licensed under a CC-BY license.

Flow Cytometry: VCAM-1/CD106 Antibody (MR106) [NBP1-43368] - Analysis using the Biotin conjugate of NBP1-43368. Staining of rat bone marrow cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Biotin (left) or 0.125 ug of Anti-Rat CD106 (VCAM-1) Biotin (right) followed by Streptavidin PE.

Flow Cytometry: VCAM-1/CD106 Antibody (MR106) [NBP1-43368] - Staining of rat bone marrow cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Rat CD106 (VCAM-1) Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE . Cells in the large scatt

Western blot analyses of protein expressions of glutathione peroxidase-1 (GPx1), vascular cell adhesion molecule 1 (VCAM-1), inducible nitric oxide (iNOS), nuclear factor (NF-kappa B), matrix metalloproteinase-9 (MMP-9), and surfactant protein A and D (SP-A and SP-D) in lung (n = 3; * and # signify P < 0.05 and P < 0.001, respectively)

• Reactivity: Rt
• Applications: WB, Flow, IHC, IP
• Clone: MR106
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

VCAM-1/CD106 Antibody (MR106) - BSA Free Summary

• Immunogen: The immunogen for this antibody was CD106.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCAM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This MR106 antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for VCAM-1/CD106 Antibody (MR106) - BSA Free

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

The MR106 monoclonal antibody reacts with rat CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and further upregulate VCAM-1 upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (NBP1-43368)(1)

Publication using NBP1-43368

• Wu NC, Liao FT, Cheng HM et al. Intravenous superoxide dismutase as a protective agent to prevent impairment of lung function induced by high tidal volume ventilation BMC Pulm Med 2017-07-26 [PMID: 28747201] (WB, Rat)

Bioinformatics

• Gene Symbol: VCAM1